In:
International Journal of Toxicology, SAGE Publications, Vol. 29, No. 3 ( 2010-05), p. 247-258
Abstract:
Chronic exposure to arylamines through diet and/or smoking has been associated with genetic changes and tumorigenesis. Cellular proliferation, apoptosis, and histological changes in liver tissue were investigated in Gambusia affinis ( G affinis) after chronic dietary exposure to 6.9 mM and 0.069 mM concentrations of benzidine (BZ), 2-aminofluorene (2AF), and their combination for 4, 8, and 12 weeks, respectively. The proliferation assay indicated non–dose-dependent increases in cellular proliferation over the controls for all treatment groups at 4 and 12 weeks but not at 8 weeks except for the low dose of 2AF. The apoptosis assay showed effects in the low-dose group of 2AF and BZ at 4 weeks only. Hematoxylin/eosin staining of liver tissue revealed an increase in oval/spindle cell proliferation and altered foci formation in the treated groups compared with controls. These results demonstrate a mammalian-like response to 2AF and BZ in G affinis liver.
Type of Medium:
Online Resource
ISSN:
1091-5818
,
1092-874X
DOI:
10.1177/1091581810363745
Language:
English
Publisher:
SAGE Publications
Publication Date:
2010
detail.hit.zdb_id:
1500682-7
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