In:
Advanced Healthcare Materials, Wiley, Vol. 3, No. 2 ( 2014-02), p. 187-196
Abstract:
Here, two types of cellulose‐based in vitro diagnostic devices are demonstrated for the diagnosis of dengue virus infection in both buffer system and human serum: 1) paper‐based ELISA for providing the semiquantitative information of the disease activity of serotype‐2 dengue fever to healthcare persons (i.e., monitoring the disease activity with a specific serotype in single patients); 2) lateral flow immunoassays to screen for infection with serotype‐2 dengue fever (i.e., rapid YES or NO diagnosis prepared for large populations, in terms of global public health). Paper‐based ELISA (specific to serotype‐2 dengue fever), which builds off of our previous studies and a revised previous ELISA procedure, owns multiple advantages: 1) high sensitivity (about 40 times higher than the current ELISA‐based approaches, due to our therapeutic‐based monoclonal antibody) and specificity (specific to dengue virus serotype‐2 nonstructural protein‐1 antigens); 2) tiny amount of sample and reagent used for single tests; 3) short operating duration (i.e., rapid diagnostic device); and, 4) inexpensiveness (appropriate for use in all developing and underdeveloped nations of the world). Due to the higher sensitivity and shorter operating duration of paper‐based ELISA (compared with conventional ELISA, and lateral flow immunoassays also performed in this study), this study has not only been able to perform the diagnosis of dengue virus serotype‐2 nonstructural protein‐1 antigens in both buffer system and human serum but also to evaluate dengue virus serotype‐2 envelope proteins in the buffer system, thus successfully achieving the first such use of these proteins as the target antigen for the development of diagnostic tools. These results provide a more comprehensive understanding for the genesis of dengue fever diagnostic tools (through antibody‐antigen recognition).
Type of Medium:
Online Resource
ISSN:
2192-2640
,
2192-2659
DOI:
10.1002/adhm.201300150
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2645585-7
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