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  • 1
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 100, No. Supplement_3 ( 2022-09-22), p. 397-398
    Abstract: Twelve steers, fitted with rumen canulae were used in a 4 x 4 Latin square design to examine the impact of the direct fed microbial Propionibacteria acidipropionici (PA) on rumen fermentation characteristics. All steers were housed together in one pen equipped with GrowSafe feed intake monitoring stations and one Greenfeed system used to estimate in vivo methane production. Steers were fed a corn silage-based diet throughout the experiment. Treatments consisted of PA administered at: 1) 0.0; 2) 1.0 x 108; 3)1.0 x 109; and 4) 1.0 x 1010 CFU∙animal-1∙day-1. Treatments were administered directly into the rumen as a single bolus dose daily. On day 7 and 14 of each period, rumen fluid was collected from each steer 2 h post treatment administration for VFA analysis and for determining in vitro fermentation characteristics. Following a 14d washout period, animal treatments were switched, and the experiment repeated. Data were analyzed as a 4 x 4 Latin square design. In vivo propionic acid molar proportions (25.4 vs 23.6±0.24) and total VFA concentrations (125.2 vs 121.3±1.87) were greater (P & lt; 0.05) in steers receiving PA compared with controls. In vitro DM disappearance (P & lt; 0.05; 63.3% vs 59.2%±1.12) and total VFA (P & lt; 0.05; 147.9 vs 145.2±1.76) were greater and methane (ml/g DMD) lesser (P & lt; 0.04; 13.1 vs 15.6±0.11) in fermentation vessels incubated with rumen fluid from animals receiving PA compared with controls. Dry matter disappearance (P & lt; 0.03) and propionic acid (P & lt; 0.04) increased linearly as dose of PA increased. In vitro Total VFA tended (P & lt; 0.08) to increase linearly and methane production tended (P & lt; 0.09) to decrease quadratically in response PA dose. In vivo methane production was similar across treatments. In conclusion, PA addition improved in vivo and in vitro rumen fermentation characteristics.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 1490550-4
    SSG: 12
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  • 2
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 99, No. Supplement_3 ( 2021-10-08), p. 289-289
    Abstract: Eighty-nine American Wagyu steers were used to evaluate the effects of dietary barley supplementation on feedlot performance. We hypothesized that barley supplementation would increase ADG compared to non-supplemented control animals. The experimental design was a randomized complete block design. Steers were blocked by initial body weight (BW) and randomly assigned within block to one of two treatments. Treatments consisted of 1) Control (no supplemental barley) and 2) Control diet + 0.9 kg∙animal-1∙d-1 of supplemental barley. Steers were housed in feedlot pens (all pens contained n = 4 steers/pen with the exception of one Control pen that contained n=5 steers; 11 replicates/treatment; experimental unit = pen) and fed a traditional American Wagyu finishing diet (DM basis: 68.42% DM, 14.33% CP; TDN: 74.76%, NEg: 1.16 Mcal/kg, 5.25% EE) for 270d. The basal diet consisted of grass hay, corn silage, cracked corn grain, soybean meal, corn distillers grain, white salt, ground limestone, and olive byproduct. Diets were fed once daily in the morning and barely was top dressed to the appropriate pens, immediately after the basal diet was delivered. Steers were individually weighed on d -1 and 0, and approximately every 28 days throughout the 270d experiment. Data were analyzed using a mixed effects model (PROC MIXED, SAS) for a completely randomized block design. Initial pen BW was used as a covariate in the statistical analysis of all performance data and significance was determined at P ≤ 0.05. Initial and final BW, ADG, DMI, and feed:gain were similar across treatments. Therefore, under the conditions of this experiment, these data suggest that barley supplementation for 270d to American Wagyu cattle did not impact overall animal performance.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2021
    detail.hit.zdb_id: 1490550-4
    SSG: 12
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  • 3
    In: Ruminants, MDPI AG, Vol. 2, No. 4 ( 2022-11-04), p. 448-455
    Abstract: Twelve 5-year-old beef steers, with an average weight of 2000 lbs., fitted with rumen canulae were used in a 4 × 4 incomplete Latin square design to examine the impact of the direct fed microbial Propionibacterium acidipropionici CP 88 (PA) on rumen fermentation characteristics, in vitro CH4, CO2, and N2 production, and in vivo CH4 and CO2 production. All steers were housed in the same pen equipped with eight GrowSafe feeding stations to monitor individual animal feed intake and one GreenFeed System to estimate individual animal CH4 and CO2 production. Steers were fed a corn-silage-based diet throughout the experiment. Treatments consisted of PA administered at: (1) control (0.0); (2) 1.0 × 108; (3) 1.0 × 109; and (4) 1.0 × 1010 cfu∙animal−1∙day (d)−1. Treatments were administered directly into the rumen as a single bolus dose daily. On day 7 and 14 of each period, rumen fluid was collected from each steer 2 h post treatment administration for VFA analysis and in vitro DM digestibility determination. Following a 14 d washout period, animal treatments were switched and the experiment repeated until the 4 × 4 Latin square was complete. In vivo propionic acid molar proportions and total VFA concentrations were greater (p 〈 0.05) in steers receiving PA when compared with controls. All other in vivo rumen fermentation characteristics were similar across treatments. In vitro DM disappearance (p 〈 0.05) and total VFA (p 〈 0.05) were greater and CH4 lesser (p 〈 0.04) in fermentation vessels incubated with rumen fluid from animals receiving PA when compared with controls. Dry matter disappearance (p 〈 0.03) and propionic acid molar proportions increased (p 〈 0.04) linearly as the dose of PA increased. In vitro total VFA tended (p 〈 0.08) to increase linearly and CH4 production per unit of DM digested tended (p 〈 0.09) to decrease quadratically in response to PA dose. All other in vitro rumen fermentation characteristics were similar across treatments. These data indicate that PA impacts in vivo and in vitro rumen fermentation characteristics.
    Type of Medium: Online Resource
    ISSN: 2673-933X
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 3136505-X
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