In:
Cytogenetic and Genome Research, S. Karger AG, Vol. 147, No. 2-3 ( 2015), p. 186-194
Abstract:
〈 i 〉 Aegilops mutica 〈 /i 〉 Boiss., a diploid species (2n = 2x = 14, TT), has been rarely studied before. In this research, a hexaploid wheat (cv. Chinese Spring)- 〈 i 〉 Ae. mutica 〈 /i 〉 partial amphiploid and a wheat- 〈 i 〉 Ae. mutica 〈 /i 〉 addition line were characterized by chromosome karyotyping, FISH using oligonucleotides Oligo-pTa535-1, Oligo-pSc119.2-1, and (GAA) 〈 sub 〉 8 〈 /sub 〉 as probes, and EST-based molecular markers. The results showed that the partial amphiploid strain consisted of 20 pairs of wheat chromosomes and 7 pairs of 〈 i 〉 Ae. mutica 〈 /i 〉 chromosomes, with both wheat 7B chromosomes missing. EST-based molecular marker data suggested that the wheat- 〈 i 〉 Ae. mutica 〈 /i 〉 addition line carries the 7T chromosome. Resistance tests indicated that both the partial amphiploid and the 7T addition line were highly resistant to powdery mildew, whereas the wheat control line Chinese Spring was highly susceptible, indicating the presence of a potentially new powdery mildew resistance gene on the 〈 i 〉 Ae. mutica 〈 /i 〉 7T chromosome. The karyotype, FISH patterns, and molecular markers can now be used to identify 〈 i 〉 Ae. mutica 〈 /i 〉 chromatin in a wheat background, and the 7T addition could be used as a new powdery mildew resistance source for wheat breeding.
Type of Medium:
Online Resource
ISSN:
1424-8581
,
1424-859X
Language:
English
Publisher:
S. Karger AG
Publication Date:
2015
detail.hit.zdb_id:
2061918-2
SSG:
12
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