In:
The Journal of Experimental Medicine, Rockefeller University Press, Vol. 215, No. 8 ( 2018-08-06), p. 2211-2226
Abstract:
Tle/Groucho proteins are transcriptional corepressors interacting with Tcf/Lef and Runx transcription factors, but their physiological roles in T cell development remain unknown. Conditional targeting of Tle1, Tle3 and Tle4 revealed gene dose–dependent requirements for Tle proteins in CD8 + lineage cells. Upon ablating all three Tle proteins, generation of CD8 + T cells was greatly diminished, largely owing to redirection of MHC-I–selected thymocytes to CD4 + lineage; the remaining CD8-positive T cells showed aberrant up-regulation of CD4 + lineage-associated genes including Cd4 , Thpok , St8sia6 , and Foxp3 . Mechanistically, Tle3 bound to Runx-occupied Thpok silencer, in post-selection double-positive thymocytes to prevent excessive ThPOK induction and in mature CD8 + T cells to silence Thpok expression. Tle3 also bound to Tcf1-occupied sites in a few CD4 + lineage-associated genes, including Cd4 silencer and St8sia6 introns, to repress their expression in mature CD8 + T cells. These findings indicate that Tle corepressors are differentially partitioned to Runx and Tcf/Lef complexes to instruct CD8 + lineage choice and cooperatively establish CD8 + T cell identity, respectively.
Type of Medium:
Online Resource
ISSN:
0022-1007
,
1540-9538
DOI:
10.1084/jem.20171514
Language:
English
Publisher:
Rockefeller University Press
Publication Date:
2018
detail.hit.zdb_id:
1477240-1
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