In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 3 ( 2021-3-30), p. e1009480-
Abstract:
Dengue virus (DENV) causes dengue fever and severe hemorrhagic fever in humans and is primarily transmitted by Aedes aegypti and A . albopictus mosquitoes. The incidence of DENV infection has been gradually increasing in recent years due to global urbanization and international travel. Understanding the virulence determinants in host and vector transmissibility of emerging epidemic DENV will be critical to combat potential outbreaks. The DENV serotype 2 (DENV-2), which caused a widespread outbreak in Taiwan in 2015 (TW2015), is of the Cosmopolitan genotype and is phylogenetically related to the virus strain linked to another large outbreak in Indonesia in 2015. We found that the TW2015 virus was highly virulent in type I and type II interferon-deficient mice, with robust replication in spleen, lung, and intestine. The TW2015 virus also had high transmissibility to Aedes mosquitoes and could be effectively spread in a continuous mosquitoes-mouse-mosquitoes-mouse transmission cycle. By making 16681-based mutants carrying different segments of the TW2015 virus, we identified the structural pre-membrane (prM) and envelope (E) genes as key virulence determinants in the host, with involvement in the high transmissibility of the TW2015 virus in mosquitoes. The transmission mouse model will make a useful platform for evaluation of DENV with high epidemic potential and development of new strategies against dengue outbreaks.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009480
DOI:
10.1371/journal.ppat.1009480.g001
DOI:
10.1371/journal.ppat.1009480.g002
DOI:
10.1371/journal.ppat.1009480.g003
DOI:
10.1371/journal.ppat.1009480.g004
DOI:
10.1371/journal.ppat.1009480.g005
DOI:
10.1371/journal.ppat.1009480.g006
DOI:
10.1371/journal.ppat.1009480.g007
DOI:
10.1371/journal.ppat.1009480.s001
DOI:
10.1371/journal.ppat.1009480.s002
DOI:
10.1371/journal.ppat.1009480.s003
DOI:
10.1371/journal.ppat.1009480.s004
DOI:
10.1371/journal.ppat.1009480.s005
DOI:
10.1371/journal.ppat.1009480.s006
DOI:
10.1371/journal.ppat.1009480.s007
DOI:
10.1371/journal.ppat.1009480.s008
DOI:
10.1371/journal.ppat.1009480.s009
DOI:
10.1371/journal.ppat.1009480.r001
DOI:
10.1371/journal.ppat.1009480.r002
DOI:
10.1371/journal.ppat.1009480.r003
DOI:
10.1371/journal.ppat.1009480.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
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