In:
Frontiers in Cellular and Infection Microbiology, Frontiers Media SA, Vol. 11 ( 2022-1-17)
Abstract:
Allergic asthma, a chronic airway inflammatory disease, is a critical public health problem. Indoor house dust mites (HDMs) could cause allergic asthma. The prevalence of sensitization to Dermatophagoides microceras ( Der m ) was approximately 80% and is related to the immunoglobulin E crossing-reactivity of mites belonging to the same genus, Dermatophagoides pteronyssinus ( Der p ) and Dermatophagoides farina ( Der f ). However, studies on Der m are scant. Methods We used integrated OMICs approaches to identify and characterize the group 2 mite allergen-like protein in Der m ( Der m 2 ). We established a Der m 2 -induced allergic asthma mouse model and treated the mice with a fungal immunomodulatory protein (FIP- fve ) isolated from Flammulina veluptipes to evaluate the allergenicity of Der m 2 and the immunomodulatory effects of FIP- fve . Results By performing de novo draft genome assembly and comparative genome analysis, we identified the putative 144-amino acid Der m 2 in silico and further confirmed its existence through liquid chromatography–tandem mass spectrometry. Der m 2 is a lipopolysaccharides (LPS)-binding protein. Thus, we examined the LPS-binding activity of recombinant Der m 2 by performing molecular docking analysis, co-immunoprecipitation (Co-IP), and a pull-down assay. Der m 2 elicited the production of pro-inflammatory cytokines, interleukin (IL)-6, and IL-8 in BEAS-2B cells, a human bronchial epithelial cell line, and induced airway hyperresponsiveness in mice. Furthermore, in mice sensitized with Der m 2 , the administration of FIP- fve in either the earlier stage or the late stage, FIP- fve alleviated allergic asthma by moderating airway inflammation and remodeling. Conclusions Der m 2 induced inflammatory responses in cell and mouse models. FIP- fve alleviated inflammation in Der m 2 -induced asthma in mice by exerting an immunomodulatory effect.
Type of Medium:
Online Resource
ISSN:
2235-2988
DOI:
10.3389/fcimb.2021.793559
DOI:
10.3389/fcimb.2021.793559.s001
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2619676-1
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