In:
Mediators of Inflammation, Hindawi Limited, Vol. 2018 ( 2018), p. 1-10
Abstract:
Our previous studies showed that γδ T cells provided immune protection against Chlamydial muridarum (Cm), an obligate intracellular strain of chlamydia trachomatis, lung infection by producing abundant IL-17. In this study, we investigated the proliferation and activation of lung γδ T cell subsets, specifically the IL-17 and IFN γ production by them following Cm lung infection. Our results found that five γδ T cell subsets, V γ 1+ T, V γ 2+ T, V γ 4+ T, V γ 5+ T, and V γ 6+ T, expressed in lungs of naïve mice, while Cm lung infection mainly induced the proliferation and activation of V γ 4+ T cells at day 3 p.i., following V γ 1+ T cells at day 7 p.i. Cytokine detection showed that Cm lung infection induced IFN γ secretion firstly by V γ 4+ T cells at very early stage (day 3) and changed to V γ 1+ T cells at midstage (day 7). Furthermore, V γ 4+ T cell is the main γδ T cell subset that secretes IL-17 at the very early stage of Cm lung infection and V γ 1+ T cell did not secrete IL-17 during the infection. These findings provide in vivo evidence that V γ 4+T cells are the major IL-17 and IFN γ -producing γδ T cell subsets at the early period of Cm lung infection.
Type of Medium:
Online Resource
ISSN:
0962-9351
,
1466-1861
DOI:
10.1155/2018/6265746
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2018
detail.hit.zdb_id:
2008065-7
Bookmarklink