In:
Journal of Separation Science, Wiley, Vol. 33, No. 4-5 ( 2010-03), p. 651-657
Abstract:
Pressurized CEC (pCEC) with UV detection was developed for the simultaneous analysis of endogenetic and ectogenic plant hormones. Effects of some crucial factors including mobile phase, buffer concentration, pH value and applied voltage on pCEC separation were investigated, respectively. Under the optimum conditions, namely a packed capillary column (3 μm Hypersil C 18 , 45 cm total length with 100 μm id×20 cm effective length) as the separation channel; ACN: 10 mmol/L phosphate (50:50, v/v, %) as the mobile phase, pH 4.0; pump flow rate 0.035 mL/min; separation voltage −10 kV; UV wavelength 222 nm, a complete separation of five hormones was accomplished. The pCEC assay presented good linearity (0.8–100 μg/mL), with LOD of 0.2 μg/mL and R in range of 0.998–0.999. Meanwhile, a lack‐of‐fit test was performed to evaluate these linear models. Results showed that no significant lack‐of‐fit was observed and these models were appropriate (α=0.05).
Type of Medium:
Online Resource
ISSN:
1615-9306
,
1615-9314
DOI:
10.1002/jssc.v33:4/5
DOI:
10.1002/jssc.200900603
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
2047990-6
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