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Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers

Holmes, Susannah ; Kirkwood, Henry J. ; Bean, Richard ; [et al.]

Springer Science and Business Media LLC ; 2022

In: Nature Communications Vol. 13, No. 1 ( 2022-08-11)

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In: Nature Communications, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2022-08-11)

Abstract: The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by 〈 1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.

Type of Medium: Online Resource

ISSN: 2041-1723

URL: Article

DOI: 10.1038/s41467-022-32434-6

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2022

detail.hit.zdb_id: 2553671-0

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The 1-Megapixel pnCCD detector for the Small Quantum Systems Instrument at the European XFEL: system and operation aspects

Kuster, Markus ; Ahmed, Karim ; Ballak, Kai-Erik ; [et al.]

International Union of Crystallography (IUCr) ; 2021

In: Journal of Synchrotron Radiation Vol. 28, No. 2 ( 2021-03-01), p. 576-587

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In: Journal of Synchrotron Radiation, International Union of Crystallography (IUCr), Vol. 28, No. 2 ( 2021-03-01), p. 576-587

Abstract: The X-ray free-electron lasers that became available during the last decade, like the European XFEL (EuXFEL), place high demands on their instrumentation. Especially at low photon energies below 1 keV, detectors with high sensitivity, and consequently low noise and high quantum efficiency, are required to enable facility users to fully exploit the scientific potential of the photon source. A 1-Megapixel pnCCD detector with a 1024 × 1024 pixel format has been installed and commissioned for imaging applications at the Nano-Sized Quantum System (NQS) station of the Small Quantum System (SQS) instrument at EuXFEL. The instrument is currently operating in the energy range between 0.5 and 3 keV and the NQS station is designed for investigations of the interaction of intense FEL pulses with clusters, nano-particles and small bio-molecules, by combining photo-ion and photo-electron spectroscopy with coherent diffraction imaging techniques. The core of the imaging detector is a pn-type charge coupled device (pnCCD) with a pixel pitch of 75 µm × 75 µm. Depending on the experimental scenario, the pnCCD enables imaging of single photons thanks to its very low electronic noise of 3 e − and high quantum efficiency. Here an overview on the EuXFEL pnCCD detector and the results from the commissioning and first user operation at the SQS experiment in June 2019 are presented. The detailed descriptions of the detector design and capabilities, its implementation at EuXFEL both mechanically and from the controls side as well as important data correction steps aim to provide useful background for users planning and analyzing experiments at EuXFEL and may serve as a benchmark for comparing and planning future endstations at other FELs.

Type of Medium: Online Resource

ISSN: 1600-5775

URL: Article

DOI: 10.1107/S1600577520015659

Language: Unknown

Publisher: International Union of Crystallography (IUCr)

Publication Date: 2021

detail.hit.zdb_id: 2021413-3

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The Karabo distributed control system

Hauf, Steffen ; Heisen, Burkhard ; Aplin, Steve ; [et al.]

International Union of Crystallography (IUCr) ; 2019

In: Journal of Synchrotron Radiation Vol. 26, No. 5 ( 2019-09-01), p. 1448-1461

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In: Journal of Synchrotron Radiation, International Union of Crystallography (IUCr), Vol. 26, No. 5 ( 2019-09-01), p. 1448-1461

Abstract: The Karabo distributed control system has been developed to address the challenging requirements of the European X-ray Free Electron Laser facility, including complex and custom-made hardware, high data rates and volumes, and close integration of data analysis for distributed processing and rapid feedback. Karabo is a pluggable, distributed application management system forming a supervisory control and data acquisition environment as part of a distributed control system. Karabo provides integrated control of hardware, monitoring, data acquisition and data analysis on distributed hardware, allowing rapid control feedback based on complex algorithms. Services exist for access control, data logging, configuration management and situational awareness through alarm indicators. The flexible framework enables quick response to the changing requirements in control and analysis, and provides an efficient environment for development, and a single interface to make all changes immediately available to operators and experimentalists.

Type of Medium: Online Resource

ISSN: 1600-5775

URL: Article

DOI: 10.1107/S1600577519006696

DOI: 10.1107/S1600577519006696/xq5007sup1.pdf

Language: Unknown

Publisher: International Union of Crystallography (IUCr)

Publication Date: 2019

detail.hit.zdb_id: 2021413-3

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A time projection chamber with GEM-based readout

Attié, David ; Behnke, Ties ; Bellerive, Alain ; [et al.]

Elsevier BV ; 2017

In: Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment Vol. 856 ( 2017-06), p. 109-118

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In: Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Elsevier BV, Vol. 856 ( 2017-06), p. 109-118

Type of Medium: Online Resource

ISSN: 0168-9002

URL: Article

DOI: 10.1016/j.nima.2016.11.002

RVK:

UA 5680.A

Language: English

Publisher: Elsevier BV

Publication Date: 2017

detail.hit.zdb_id: 1466532-3

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MHz data collection of a microcrystalline mixture of different jack bean proteins

Grünbein, Marie Luise ; Bielecki, Johan ; Gorel, Alexander ; [et al.]

Springer Science and Business Media LLC ; 2019

In: Scientific Data Vol. 6, No. 1 ( 2019-04-03)

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In: Scientific Data, Springer Science and Business Media LLC, Vol. 6, No. 1 ( 2019-04-03)

Abstract: We provide a detailed description of a serial femtosecond crystallography (SFX) dataset collected at the European X-ray free-electron laser facility (EuXFEL). The EuXFEL is the first high repetition rate XFEL delivering MHz X-ray pulse trains at 10 Hz. The short spacing ( 〈 1 µs) between pulses requires fast flowing microjets for sample injection and high frame rate detectors. A data set was recorded of a microcrystalline mixture of at least three different jack bean proteins (urease, concanavalin A, concanavalin B). A one megapixel Adaptive Gain Integrating Pixel Detector (AGIPD) was used which has not only a high frame rate but also a large dynamic range. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development and for data analysis training for prospective XFEL users.

Type of Medium: Online Resource

ISSN: 2052-4463

URL: Article

DOI: 10.1038/s41597-019-0010-0

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2019

detail.hit.zdb_id: 2775191-0

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Megahertz data collection from protein microcrystals at an X-ray free-electron laser

Grünbein, Marie Luise ; Bielecki, Johan ; Gorel, Alexander ; [et al.]

Springer Science and Business Media LLC ; 2018

In: Nature Communications Vol. 9, No. 1 ( 2018-08-28)

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In: Nature Communications, Springer Science and Business Media LLC, Vol. 9, No. 1 ( 2018-08-28)

Abstract: X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates of only 10–120 Hz, placing significant demands on beam time and sample consumption. We describe serial femtosecond crystallography experiments performed at the European XFEL, the first MHz repetition rate XFEL, delivering 1.128 MHz X-ray pulse trains at 10 Hz. Given the short spacing between pulses, damage caused by shock waves launched by one XFEL pulse on sample probed by subsequent pulses is a concern. To investigate this issue, we collected data from lysozyme microcrystals, exposed to a ~15 μm XFEL beam. Under these conditions, data quality is independent of whether the first or subsequent pulses of the train were used for data collection. We also analyzed a mixture of microcrystals of jack bean proteins, from which the structure of native, magnesium-containing concanavalin A was determined.

Type of Medium: Online Resource

ISSN: 2041-1723

URL: Article

DOI: 10.1038/s41467-018-05953-4

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2018

detail.hit.zdb_id: 2553671-0

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Endovascular thrombectomy for acute ischaemic stroke with established large infarct: multicentre, open-label, randomised trial

Bendszus, Martin ; Fiehler, Jens ; Subtil, Fabien ; [et al.]

Elsevier BV ; 2023

In: The Lancet Vol. 402, No. 10414 ( 2023-11), p. 1753-1763

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In: The Lancet, Elsevier BV, Vol. 402, No. 10414 ( 2023-11), p. 1753-1763

Type of Medium: Online Resource

ISSN: 0140-6736

URL: Article

DOI: 10.1016/S0140-6736(23)02032-9

RVK:

XA 10000

Language: English

Publisher: Elsevier BV

Publication Date: 2023

detail.hit.zdb_id: 2067452-1

detail.hit.zdb_id: 3306-6

detail.hit.zdb_id: 1476593-7

SSG: 5,21

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