In:
Biochemical Journal, Portland Press Ltd., Vol. 299, No. 1 ( 1994-04-01), p. 85-90
Abstract:
The effects of u.v. A radiations on phospholipid synthesis were studied in the N.C.T.C. 2544 human keratinocyte cell line, by using [14C]arachidonic acid, [14C] oleic acid or sodium [32P]orthophosphate as precursors. Cells were irradiated in Hanks' medium with 365 nm light at doses up to 19 J/cm2, and then phospholipid synthesis from the three precursors was studied. Under these conditions, only small alterations in the incorporation pattern of [14C] arachidonic into phospholipids [phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI)] were observed, for u.v. A irradiation doses up to 19 J/cm2. In contrast, with [14C] oleic acid as precursor, two additional spots were observed, which co-migrate with pure phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG) standards. The incorporation of [14C]oleic acid into PG and DPG was decreased in a dose-dependent manner after u.v. A exposure, with about 50% and 75% decreases at 9.5 J/cm2 and 19 J/cm2 respectively. As for arachidonic acid incorporation, no significant differences in the synthesis of the major phospholipids (PC, PE, PI) were noted upon u.v. A exposure. The dramatic and selective decrease in PG and DPG syntheses was confirmed with [32P] orthophosphate as precursor. As DPG is a specific component of the mitochondrial inner membrane, it appears that one of the early kinds of damage induced by u.v. A irradiation could be the impairment of mitochondrial functions.
Type of Medium:
Online Resource
ISSN:
0264-6021
,
1470-8728
Language:
English
Publisher:
Portland Press Ltd.
Publication Date:
1994
detail.hit.zdb_id:
1473095-9
SSG:
12
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