In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 8 ( 2021-8-11), p. e1009758-
Abstract:
Since the pandemic of COVID-19 has intensely struck human society, small animal model for this infectious disease is in urgent need for basic and pharmaceutical research. Although several COVID-19 animal models have been identified, many of them show either minimal or inadequate pathophysiology after SARS-CoV-2 challenge. Here, we describe a new and versatile strategy to rapidly establish a mouse model for emerging infectious diseases in one month by multi-route, multi-serotype transduction with recombinant adeno-associated virus (AAV) vectors expressing viral receptor. In this study, the proposed approach enables profound and enduring systemic expression of SARS-CoV-2-receptor hACE2 in wild-type mice and renders them vulnerable to SARS-CoV-2 infection. Upon virus challenge, generated AAV/hACE2 mice showed pathophysiology closely mimicking the patients with severe COVID-19. The efficacy of a novel therapeutic antibody cocktail RBD-chAbs for COVID-19 was tested and confirmed by using this AAV/hACE2 mouse model, further demonstrating its successful application in drug development.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009758
DOI:
10.1371/journal.ppat.1009758.g001
DOI:
10.1371/journal.ppat.1009758.g002
DOI:
10.1371/journal.ppat.1009758.g003
DOI:
10.1371/journal.ppat.1009758.g004
DOI:
10.1371/journal.ppat.1009758.g005
DOI:
10.1371/journal.ppat.1009758.g006
DOI:
10.1371/journal.ppat.1009758.s001
DOI:
10.1371/journal.ppat.1009758.s002
DOI:
10.1371/journal.ppat.1009758.s003
DOI:
10.1371/journal.ppat.1009758.s004
DOI:
10.1371/journal.ppat.1009758.s005
DOI:
10.1371/journal.ppat.1009758.s006
DOI:
10.1371/journal.ppat.1009758.s007
DOI:
10.1371/journal.ppat.1009758.s008
DOI:
10.1371/journal.ppat.1009758.s009
DOI:
10.1371/journal.ppat.1009758.s010
DOI:
10.1371/journal.ppat.1009758.s011
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
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