In:
Combinatorial Chemistry & High Throughput Screening, Bentham Science Publishers Ltd., Vol. 26, No. 10 ( 2023-08), p. 1856-1865
Abstract:
Chronic prostatitis (CP) is one of the most common
diseases in young and middle-aged men but lacks effective treatment. Shuangshi Tonglin Capsule (SSTLC) is a clinical drug for the treatment of chronic prostatitis. However, the underlying molecular
mechanisms of SSTLC in treating CP are still unclear. In this study, we researched the underlying mechanisms of SSTLC in treating chronic prostatitis. Methods: The ingredients of SSTLC were received from the TCMSP and BATMAN databases,
and the CP targets were collected based on GeneCards and OMIM. Then, the PPI network and the “drug-ingredient-target” network map were constructed. GO and KEGG enrichment analyses by
using DAVID. Molecular docking was performed by using AutoDock 4.2 and PyMol. And using animal experiments to verify the potential effect of SSTLC in CP. Results: SSTLC contained 10 herbs, 158 chemical ingredients and 277 targets, 2002, diseaserelated
targets were obtained. Network analysis outcomes indicated that VEGFA, TNF, MAPK1, EGFR, and MAPK8 are the key targets of SSTLC in treating chronic prostatitis. Furthermore, molecular
docking revealed that quercetin, luteolin, and kaempferol exhibited a strong binding effect. Animal experimental indicated that SSTLC can reduce the pathological damage to prostate tissue.
And, we found that high-dose SSTLC significantly reduced the level of TNF-α and downregulated the expression of EGFR, p-p38 and p-ERK1/2 (P 〈 0.05). Conclusions: This study determined the pharmacological effects of SSTLC and the potential mechanism
of action on SSTLC to treat CP, it provides a new idea for traditional Chinese medicine to treat chronic prostatitis.
Type of Medium:
Online Resource
ISSN:
1386-2073
DOI:
10.2174/1386207326666221024144543
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2023
SSG:
15,3
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