In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 9 ( 2021-9-23), p. e1009566-
Abstract:
The hemagglutinin (HA) of A/H3N2 pandemic influenza viruses (IAVs) of 1968 differed from its inferred avian precursor by eight amino acid substitutions. To determine their phenotypic effects, we studied recombinant variants of A/Hong Kong/1/1968 virus containing either human-type or avian-type amino acids in the corresponding positions of HA. The precursor HA displayed receptor binding profile and high conformational stability typical for duck IAVs. Substitutions Q226L and G228S, in addition to their known effects on receptor specificity and replication, marginally decreased HA stability. Substitutions R62I, D63N, D81N and N193S reduced HA binding avidity. Substitutions R62I, D81N and A144G promoted viral replication in human airway epithelial cultures. Analysis of HA sequences revealed that substitutions D63N and D81N accompanied by the addition of N-glycans represent common markers of avian H3 HA adaptation to mammals. Our results advance understanding of genotypic and phenotypic changes in IAV HA required for avian-to-human adaptation and pandemic emergence.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009566
DOI:
10.1371/journal.ppat.1009566.g001
DOI:
10.1371/journal.ppat.1009566.g002
DOI:
10.1371/journal.ppat.1009566.g003
DOI:
10.1371/journal.ppat.1009566.g004
DOI:
10.1371/journal.ppat.1009566.g005
DOI:
10.1371/journal.ppat.1009566.g006
DOI:
10.1371/journal.ppat.1009566.g007
DOI:
10.1371/journal.ppat.1009566.g008
DOI:
10.1371/journal.ppat.1009566.g009
DOI:
10.1371/journal.ppat.1009566.g010
DOI:
10.1371/journal.ppat.1009566.t001
DOI:
10.1371/journal.ppat.1009566.t002
DOI:
10.1371/journal.ppat.1009566.t003
DOI:
10.1371/journal.ppat.1009566.s001
DOI:
10.1371/journal.ppat.1009566.s002
DOI:
10.1371/journal.ppat.1009566.s003
DOI:
10.1371/journal.ppat.1009566.s004
DOI:
10.1371/journal.ppat.1009566.s005
DOI:
10.1371/journal.ppat.1009566.s006
DOI:
10.1371/journal.ppat.1009566.s007
DOI:
10.1371/journal.ppat.1009566.s008
DOI:
10.1371/journal.ppat.1009566.s009
DOI:
10.1371/journal.ppat.1009566.s010
DOI:
10.1371/journal.ppat.1009566.s011
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
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