In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 20, No. 3 ( 2024-3-5), p. e1012060-
Abstract:
The recent discovery of Hepatitis D (HDV) -like viruses across a wide range of taxa led to the establishment of the Kolmioviridae family. Recent studies suggest that kolmiovirids can be satellites of viruses other than Hepatitis B virus (HBV), challenging the strict HBV/HDV-association dogma. Studying whether kolmiovirids are able to replicate in any animal cell they enter is essential to assess their zoonotic potential. Here, we compared replication of three kolmiovirids: HDV, rodent (RDeV) and snake (SDeV) deltavirus in vitro and in vivo . We show that SDeV has the narrowest and RDeV the broadest host cell range. High resolution imaging of cells persistently replicating these viruses revealed nuclear viral hubs with a peculiar RNA-protein organization. Finally, in vivo hydrodynamic delivery of viral replicons showed that both HDV and RDeV, but not SDeV, efficiently replicate in mouse liver, forming massive nuclear viral hubs. Our comparative analysis lays the foundation for the discovery of specific host factors controlling Kolmioviridae host-shifting.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1012060
DOI:
10.1371/journal.ppat.1012060.g001
DOI:
10.1371/journal.ppat.1012060.g002
DOI:
10.1371/journal.ppat.1012060.g003
DOI:
10.1371/journal.ppat.1012060.g004
DOI:
10.1371/journal.ppat.1012060.g005
DOI:
10.1371/journal.ppat.1012060.g006
DOI:
10.1371/journal.ppat.1012060.s001
DOI:
10.1371/journal.ppat.1012060.s002
DOI:
10.1371/journal.ppat.1012060.s003
DOI:
10.1371/journal.ppat.1012060.s004
DOI:
10.1371/journal.ppat.1012060.s005
DOI:
10.1371/journal.ppat.1012060.s006
DOI:
10.1371/journal.ppat.1012060.s007
DOI:
10.1371/journal.ppat.1012060.s008
DOI:
10.1371/journal.ppat.1012060.s009
DOI:
10.1371/journal.ppat.1012060.s010
DOI:
10.1371/journal.ppat.1012060.s011
DOI:
10.1371/journal.ppat.1012060.s012
DOI:
10.1371/journal.ppat.1012060.s013
DOI:
10.1371/journal.ppat.1012060.s014
DOI:
10.1371/journal.ppat.1012060.s015
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2024
detail.hit.zdb_id:
2205412-1
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