In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 1 ( 2021-1-12), p. e0243292-
Abstract:
Two winter rapeseed cultivars, “NS” (cold tolerant) and “NF” (cold sensitive), were used to reveal the morphological, physiological, and proteomic characteristics in leaves of plants after treatment at -4°C for 12 h(T1) and 24 h(T2), and at room temperature(T0), to understand the molecular mechanisms of cold tolerance. Antioxidant activity and osmotic adjustment ability were higher, and plasma membrane injury was less obvious, in NS than in NF under cold stress. We detected different abundant proteins (DAPs) related to cold tolerance in winter rapeseed through data-independent acquisition (DIA). Compared with NF, A total of 1,235 and 1,543 DAPs were identified in the NSs under T1 and T2, respectively. Compared with NF, 911 proteins were more abundant in NS only after cold treatment. Some of these proteins were related to ROS scavenging through four metabolic pathways: lysine degradation; phenylalanine, tyrosine, and tryptophan; flavonoid biosynthesis; and ubiquinone and other terpenoid-quinone biosynthesis. Analysis of these proteins in the four candidate pathways revealed that they were rapidly accumulated to quickly enhance ROS scavenging and improve the cold tolerance of NS. These proteins were noticeably more abundant during the early stage of cold stress, which was critical for avoiding ROS damage.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0243292
DOI:
10.1371/journal.pone.0243292.g001
DOI:
10.1371/journal.pone.0243292.g002
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10.1371/journal.pone.0243292.g003
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10.1371/journal.pone.0243292.g004
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10.1371/journal.pone.0243292.g005
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10.1371/journal.pone.0243292.g006
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10.1371/journal.pone.0243292.g007
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10.1371/journal.pone.0243292.t001
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10.1371/journal.pone.0243292.s001
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10.1371/journal.pone.0243292.s002
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10.1371/journal.pone.0243292.s003
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10.1371/journal.pone.0243292.s004
DOI:
10.1371/journal.pone.0243292.s005
DOI:
10.1371/journal.pone.0243292.s006
DOI:
10.1371/journal.pone.0243292.s007
DOI:
10.1371/journal.pone.0243292.s008
DOI:
10.1371/journal.pone.0243292.r001
DOI:
10.1371/journal.pone.0243292.r002
DOI:
10.1371/journal.pone.0243292.r003
DOI:
10.1371/journal.pone.0243292.r004
DOI:
10.1371/journal.pone.0243292.r005
DOI:
10.1371/journal.pone.0243292.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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