In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 9 ( 2021-9-3), p. e0256875-
Abstract:
Coix lacryma-jobi L. is a very important economic crop widely cultivated in Southeast Asia. Drought affects more than four million square kilometers every year, and is a significant factor limiting agricultural productivity. However, relatively little is known about how Coix lacryma-jobi L. responds to drought treatments. To obtain a detailed and comprehensive understanding of the mechanisms regulating the transcriptional responses of Coix lacryma-jobi L. to drought treatment, we employed high throughput short-read sequencing of cDNA prepared from polyadenylated RNA to explore global gene expression after a seven-day drought treatment. We generated a de novo assembled transcriptome comprising 65,480 unique sequences. Differential expression analysis based on RSEM-estimated transcript abundances identified 5,315 differentially expressed genes (DEGs) when comparing samples from plants following drought-treatment and from the appropriate controls. Among these, the transcripts for 3,460 genes were increased in abundance, whereas 1,855 were decreased. Real-time quantitative PCR for 5 transcripts confirmed the changes identified by RNA-Seq. The results provide a transcriptional overview of the changes in Coix lacryma-jobi L. in response to drought, and will be very useful for studying the function of associated genes and selection of molecular marker of Coix lacryma-jobi L in the future.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0256875
DOI:
10.1371/journal.pone.0256875.g001
DOI:
10.1371/journal.pone.0256875.g002
DOI:
10.1371/journal.pone.0256875.g003
DOI:
10.1371/journal.pone.0256875.g004
DOI:
10.1371/journal.pone.0256875.g005
DOI:
10.1371/journal.pone.0256875.g006
DOI:
10.1371/journal.pone.0256875.g007
DOI:
10.1371/journal.pone.0256875.g008
DOI:
10.1371/journal.pone.0256875.g009
DOI:
10.1371/journal.pone.0256875.g010
DOI:
10.1371/journal.pone.0256875.t001
DOI:
10.1371/journal.pone.0256875.s001
DOI:
10.1371/journal.pone.0256875.s002
DOI:
10.1371/journal.pone.0256875.s003
DOI:
10.1371/journal.pone.0256875.s004
DOI:
10.1371/journal.pone.0256875.s005
DOI:
10.1371/journal.pone.0256875.s006
DOI:
10.1371/journal.pone.0256875.s007
DOI:
10.1371/journal.pone.0256875.s008
DOI:
10.1371/journal.pone.0256875.s009
DOI:
10.1371/journal.pone.0256875.s010
DOI:
10.1371/journal.pone.0256875.s011
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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