In:
PLOS Biology, Public Library of Science (PLoS), Vol. 20, No. 1 ( 2022-1-18), p. e3001507-
Abstract:
Genome editing can introduce designed mutations into a target genomic site. Recent research has revealed that it can also induce various unintended events such as structural variations, small indels, and substitutions at, and in some cases, away from the target site. These rearrangements may result in confounding phenotypes in biomedical research samples and cause a concern in clinical or agricultural applications. However, current genotyping methods do not allow a comprehensive analysis of diverse mutations for phasing and mosaic variant detection. Here, we developed a genotyping method with an on-target site analysis software named Determine Allele mutations and Judge Intended genotype by Nanopore sequencer (DAJIN) that can automatically identify and classify both intended and unintended diverse mutations, including point mutations, deletions, inversions, and cis double knock-in at single-nucleotide resolution. Our approach with DAJIN can handle approximately 100 samples under different editing conditions in a single run. With its high versatility, scalability, and convenience, DAJIN-assisted multiplex genotyping may become a new standard for validating genome editing outcomes.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001507
DOI:
10.1371/journal.pbio.3001507.g001
DOI:
10.1371/journal.pbio.3001507.g002
DOI:
10.1371/journal.pbio.3001507.g003
DOI:
10.1371/journal.pbio.3001507.g004
DOI:
10.1371/journal.pbio.3001507.g005
DOI:
10.1371/journal.pbio.3001507.g006
DOI:
10.1371/journal.pbio.3001507.s001
DOI:
10.1371/journal.pbio.3001507.s002
DOI:
10.1371/journal.pbio.3001507.s003
DOI:
10.1371/journal.pbio.3001507.s004
DOI:
10.1371/journal.pbio.3001507.s005
DOI:
10.1371/journal.pbio.3001507.s006
DOI:
10.1371/journal.pbio.3001507.s007
DOI:
10.1371/journal.pbio.3001507.s008
DOI:
10.1371/journal.pbio.3001507.s009
DOI:
10.1371/journal.pbio.3001507.s010
DOI:
10.1371/journal.pbio.3001507.s011
DOI:
10.1371/journal.pbio.3001507.s012
DOI:
10.1371/journal.pbio.3001507.s013
DOI:
10.1371/journal.pbio.3001507.s014
DOI:
10.1371/journal.pbio.3001507.s015
DOI:
10.1371/journal.pbio.3001507.s016
DOI:
10.1371/journal.pbio.3001507.s017
DOI:
10.1371/journal.pbio.3001507.s018
DOI:
10.1371/journal.pbio.3001507.s019
DOI:
10.1371/journal.pbio.3001507.s020
DOI:
10.1371/journal.pbio.3001507.s021
DOI:
10.1371/journal.pbio.3001507.s022
DOI:
10.1371/journal.pbio.3001507.s023
DOI:
10.1371/journal.pbio.3001507.s024
DOI:
10.1371/journal.pbio.3001507.s025
DOI:
10.1371/journal.pbio.3001507.s026
DOI:
10.1371/journal.pbio.3001507.s027
DOI:
10.1371/journal.pbio.3001507.s028
DOI:
10.1371/journal.pbio.3001507.s029
DOI:
10.1371/journal.pbio.3001507.s030
DOI:
10.1371/journal.pbio.3001507.s031
DOI:
10.1371/journal.pbio.3001507.s032
DOI:
10.1371/journal.pbio.3001507.s033
DOI:
10.1371/journal.pbio.3001507.s034
DOI:
10.1371/journal.pbio.3001507.s035
DOI:
10.1371/journal.pbio.3001507.s036
DOI:
10.1371/journal.pbio.3001507.s037
DOI:
10.1371/journal.pbio.3001507.s038
DOI:
10.1371/journal.pbio.3001507.s039
DOI:
10.1371/journal.pbio.3001507.s040
DOI:
10.1371/journal.pbio.3001507.s041
DOI:
10.1371/journal.pbio.3001507.s042
DOI:
10.1371/journal.pbio.3001507.s043
DOI:
10.1371/journal.pbio.3001507.r001
DOI:
10.1371/journal.pbio.3001507.r002
DOI:
10.1371/journal.pbio.3001507.r003
DOI:
10.1371/journal.pbio.3001507.r004
DOI:
10.1371/journal.pbio.3001507.r005
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2126773-X
Bookmarklink