In:
PLOS Computational Biology, Public Library of Science (PLoS), Vol. 17, No. 4 ( 2021-4-19), p. e1008887-
Abstract:
Mass Based Imaging (MBI) technologies such as Multiplexed Ion Beam Imaging by time of flight (MIBI-TOF) and Imaging Mass Cytometry (IMC) allow for the simultaneous measurement of the expression levels of 40 or more proteins in biological tissue, providing insight into cellular phenotypes and organization in situ . Imaging artifacts, resulting from the sample, assay or instrumentation complicate downstream analyses and require correction by domain experts. Here, we present MBI Analysis User Interface (MAUI), a series of graphical user interfaces that facilitate this data pre-processing, including the removal of channel crosstalk, noise and antibody aggregates. Our software streamlines these steps and accelerates processing by enabling real-time and interactive parameter tuning across multiple images.
Type of Medium:
Online Resource
ISSN:
1553-7358
DOI:
10.1371/journal.pcbi.1008887
DOI:
10.1371/journal.pcbi.1008887.g001
DOI:
10.1371/journal.pcbi.1008887.g002
DOI:
10.1371/journal.pcbi.1008887.g003
DOI:
10.1371/journal.pcbi.1008887.g004
DOI:
10.1371/journal.pcbi.1008887.g005
DOI:
10.1371/journal.pcbi.1008887.s001
DOI:
10.1371/journal.pcbi.1008887.s002
DOI:
10.1371/journal.pcbi.1008887.s003
DOI:
10.1371/journal.pcbi.1008887.s004
DOI:
10.1371/journal.pcbi.1008887.r001
DOI:
10.1371/journal.pcbi.1008887.r002
DOI:
10.1371/journal.pcbi.1008887.r003
DOI:
10.1371/journal.pcbi.1008887.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2193340-6
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