In:
Bulletin de l'Académie Vétérinaire de France, PERSEE Program, Vol. 156, No. 2 ( 2003), p. 41-45
Abstract:
Dissemination of Leishmania : a parasite sampled and picked up by the sandfly in the dermis of its vertebrate host. Study of an experimental model : Leishmania major and C57Bl/6 mouse. Parasites establish with their host a series of dynamic interactions, clinically visible or asymptomatic, on which depends their durability. Leishmania major is a protozoan parasite dependent on two hosts : (a) a hemophage and telmophage insect, the sandfly, and (b) a rodent. Following the inoculation, when and where do the Leishmania in their amastigote form migrate from the initial site ? In our laboratory, an infection model was developed based on current knowledge on hemophagia in telmophage sandflies. We prepared 1000 metacyclic promastigote Leishmania and inoculated them to C57Bl/6 mice via intradermal injections in the centre of the right pinna. This study, based on real-time/ quantitative PCR (qPCR) to quantify kinetoplastic DNA (kDNA), has shown that Leishmania were established in the centre of the inoculated pinna. On day 4 after the injection, we detected Leishmania kDNA in the periphery of that pinna and in the draining lymph node. The parasitic load grew regularly until day 28, then decreased between days 28 and 49. The maximum value recorded in the centre of the ear was 10,000 parasites on day 28, less in the periphery. The skin of the tail was the distant skin site where the detection of kinetoplastic kDNA was most consistent, starting on day 4. These results confirm the data on the kinetics of the parasitic load at the injection site. They also support the presence, within days after the inoculation, of a few parasites in asymptomatic distant cutaneous sites. The technique of qPCR to detect asymptomatic carriage has several applications both in Veterinary Medicine and in Public Health. The potential implications of these results on the parasite cycle are also emphasized.
Type of Medium:
Online Resource
ISSN:
0001-4192
Language:
French
Publisher:
PERSEE Program
Publication Date:
2003
detail.hit.zdb_id:
2840771-4
SSG:
22
Bookmarklink
