In:
The International Journal of Biological Markers, SAGE Publications, Vol. 28, No. 3 ( 2013-07), p. 267-273
Abstract:
In colorectal cancer, CD133 + cells from fresh biopsies proved to be more tumorigenic than their CD133 – counterparts. Nevertheless, the function of CD133 protein in tumorigenic cells seems only marginal. Moreover, CD133 expression alone is insufficient to isolate true cancer stem cells, since only 1 out of 262 CD133 + cells actually displays stem-cell capacity. Thus, new markers for colorectal cancer stem cells are needed. Here, we show the extensive characterization of CD133 + cells in 5 different colon carcinoma continuous cell lines (HT29, HCT116, Caco2, GEO and LS174T), each representing a different maturation level of colorectal cancer cells. Markers associated with stemness, tumorigenesis and metastatic potential were selected. We identified 6 molecules consistently present on CD133 + cells: CD9, CD29, CD49b, CD59, CD151, and CD326. By contrast, CD24, CD26, CD54, CD66c, CD81, CD90, CD99, CD112, CD164, CD166, and CD200 showed a discontinuous behavior, which led us to identify cell type-specific surface antigen mosaics. Finally, some antigens, e.g. CD227, indicated the possibility of classifying the CD133 + cells into 2 subsets likely exhibiting specific features. This study reports, for the first time, an extended characterization of the CD133 + cells in colon carcinoma cell lines and provides a “dictionary” of antigens to be used in colorectal cancer research.
Type of Medium:
Online Resource
ISSN:
1724-6008
,
1724-6008
Language:
English
Publisher:
SAGE Publications
Publication Date:
2013
detail.hit.zdb_id:
1475778-3
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