In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_12 ( 2015-12-01), p. 4479-4487
Abstract:
An aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2 T , was isolated from geothermal soil at Waikite, New Zealand. Strain WKT50.2 T grew at 53–76 °C and at pH 5.9–8.2. The DNA G+C content was 58.4 mol%. The major fatty acids were 12-methyl C 18 : 0 and C 18 : 0 . Polar lipids were all linked to long-chain 1,2-diols, and comprised 2-acylalkyldiol-1- O -phosphoinositol (diolPI), 2-acylalkyldiol-1- O -phosphoacylmannoside (diolP-acylMan), 2-acylalkyldiol-1- O -phosphoinositol acylmannoside (diolPI-acylMan) and 2-acylalkyldiol-1- O -phosphoinositol mannoside (diolPI-Man). Strain WKT50.2 T utilized a range of cellulosic substrates, alcohols and organic acids for growth, but was unable to utilize monosaccharides. Robust growth of WKT50.2 T was observed on protein derivatives. WKT50.2 T was sensitive to ampicillin, chloramphenicol, kanamycin, neomycin, polymyxin B, streptomycin and vancomycin. Metronidazole, lasalocid A and trimethoprim stimulated growth. Phylogenetic analysis of 16S rRNA gene sequences showed that WKT50.2 T belonged to the class Thermomicrobia within the phylum Chloroflexi , and was most closely related to Thermorudis peleae KI4 T (99.6% similarity). DNA–DNA hybridization between WKT50.2 T and Thermorudis peleae DSM 27169 T was 18.0%. Physiological and biochemical tests confirmed the phenotypic and genotypic differentiation of strain WKT50.2 T from Thermorudis peleae KI4 T and other members of the Thermomicrobia . On the basis of its phylogenetic position and phenotypic characteristics, we propose that strain WKT50.2 T represents a novel species, for which the name Thermorudis pharmacophila sp. nov. is proposed, with the type strain WKT50.2 T ( = DSM 26011 T = ICMP 20042 T ). Emended descriptions of Thermomicrobium roseum , Thermomicrobium carboxidum , Thermorudis peleae and Sphaerobacter thermophilus are also proposed, and include the description of a novel respiratory quinone, MK-8 2,3-epoxide (23%), in Thermomicrobium roseum .
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.000598
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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