In:
Chemotherapy, S. Karger AG, Vol. 49, No. 1-2 ( 2003), p. 56-61
Abstract:
We determined the susceptibilities of recent clinical isolates of 〈 i 〉 Streptococcus pneumoniae 〈 /i 〉 to 19 antibiotics. The frequency of erythromycin nonsusceptibility was high, i.e. 8/13 (61.5%), 10/14 (71.4%) and 11/11 isolates (100%) from 13 penicillin-susceptible, 14 penicillin-intermediate and 11 penicillin-resistant 〈 i 〉 S. pneumoniae 〈 /i 〉 , respectively. Macrolide resistance was detected by polymerase chain reaction (PCR) and disk diffusion methods. Of these erythromycin-nonsusceptible pneumococcal isolates, 13/29 (44.8%) isolates contained genomic copies of 〈 i 〉 mefA 〈 /i 〉 and showed non-‘D’-shaped zones of inhibition observed around rokitamycin and/or clindamycin disks. Sixteen out of 29 isolates (55.2%) contained copies of 〈 i 〉 ermB 〈 /i 〉 and showed typical ‘D’-shaped zones of inhibition, except one isolate. Although the macrolide resistance determinants, 〈 i 〉 mefA 〈 /i 〉 and 〈 i 〉 ermB 〈 /i 〉 , could be identified by PCR and disk diffusion methods, PCR methods were more reliable in elucidating these determinants. The susceptibility pattern to 14-, 15- and 16-membered macrolides and clindamycin differed between the 〈 i 〉 mefA 〈 /i 〉 + and 〈 i 〉 ermB 〈 /i 〉 + isolates. All isolates were susceptible to levofloxacin, sparfloxacin and vancomycin. The MICs of sitafloxacin were lowest among the fluoroquinolones examined for 38 isolates.
Type of Medium:
Online Resource
ISSN:
0009-3157
,
1421-9794
Language:
English
Publisher:
S. Karger AG
Publication Date:
2003
detail.hit.zdb_id:
1482111-4
SSG:
15,3
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