In:
The Journal of Immunology, The American Association of Immunologists, Vol. 196, No. 1_Supplement ( 2016-05-01), p. 46.14-46.14
Abstract:
Vaccines are a powerful tool for preventing the transmission of diseases, however very few take advantage of specific mechanistic knowledge. In order for newer and better vaccines to be developed, a greater understanding of adjuvant mechanism and immune system priming needs to be achieved. Our laboratory has focused on the natural adjuvant, PorB, a pore forming protein isolated from Neisseria meningitidis. This protein has been shown to be a TLR2 agonist with potent adjuvant activity. PorB also increases antigen-specific antibody titers and T cell proliferation when co-administered with an antigen. Hypothesis In this study, we hypothesized that PorB increases intracellular trafficking of antigen, ovalbumin (OVA), faster than OVA alone in bone marrow derived dendritic cells (BMDCs). Methods Isolated BMDC from BL6 mice were divided into 3 groups – PBS control, fluorescently labeled OVA (used antigen control), and OVA+PorB. Stimulation lasted 0, 0.5, 1, 2, or 4 hours. Cells were then stained with early endosome antigen 1 (EEA1) and lysosome-associated membrane protein 1 (LAMP1) to track the labeled OVA. Results This experiment showed that OVA uptake was increased within BMDCs stimulated with PorB. Specifically, OVA co-localized with EEA1 and LAMP1 more frequently than OVA alone. OVA co-localization was also present earlier in stimulation when PorB was present compared to OVA alone. Conclusion These insights of how BMDCs traffic antigen with adjuvants can allow for more efficient antigen presentation, lymphocyte responses, and, overall, better vaccines.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.196.Supp.46.14
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2016
detail.hit.zdb_id:
1475085-5
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