In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 15, No. 12 ( 2021-12-13), p. e0010017-
Abstract:
The epidemiology of typhoid fever in Lao People`s Democratic Republic is poorly defined. Estimating the burden of typhoid fever in endemic countries is complex due to the cost and limitations of population-based surveillance; serological approaches may be a more cost-effective alternative. ELISAs were performed on 937 serum samples (317 children and 620 adults) from across Lao PDR to measure IgG antibody titers against Vi polysaccharide and the experimental protein antigens, CdtB and HlyE. We measured the significance of the differences between antibody titers in adults and children and fitted models to assess the relationship between age and antibody titers. The median IgG titres of both anti-HylE and CdtB were significantly higher in children compared to adults (anti-HylE; 351.7 ELISA Units (EU) vs 198.1 EU, respectively; p 〈 0.0001 and anti-CdtB; 52.6 vs 12.9 EU; p 〈 0.0001). Conversely, the median anti-Vi IgG titer was significantly higher in adults than children (11.3 vs 3.0 U/ml; p 〈 0.0001). A non-linear trend line fitted to the anti-CdtB and anti-HlyE IgG data identified a peak in antibody concentration in children 〈 5 years of age. We identified elevated titers of anti-HlyE and anti-CdtB IgG in the serum of children residing in Lao PDR in comparison to adults. These antigens are associated with seroconversion after typhoid fever and may be a superior measure of disease burden than anti-Vi IgG. This approach is scalable and may be developed to assess the burden of typhoid fever in countries where the disease may be endemic, and evidence is required for the introduction of typhoid vaccines.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0010017
DOI:
10.1371/journal.pntd.0010017.g001
DOI:
10.1371/journal.pntd.0010017.g002
DOI:
10.1371/journal.pntd.0010017.g003
DOI:
10.1371/journal.pntd.0010017.g004
DOI:
10.1371/journal.pntd.0010017.t001
DOI:
10.1371/journal.pntd.0010017.t002
DOI:
10.1371/journal.pntd.0010017.s001
DOI:
10.1371/journal.pntd.0010017.s002
DOI:
10.1371/journal.pntd.0010017.s003
DOI:
10.1371/journal.pntd.0010017.s004
DOI:
10.1371/journal.pntd.0010017.s005
DOI:
10.1371/journal.pntd.0010017.s006
DOI:
10.1371/journal.pntd.0010017.s007
DOI:
10.1371/journal.pntd.0010017.s008
DOI:
10.1371/journal.pntd.0010017.s009
DOI:
10.1371/journal.pntd.0010017.s010
DOI:
10.1371/journal.pntd.0010017.s011
DOI:
10.1371/journal.pntd.0010017.r001
DOI:
10.1371/journal.pntd.0010017.r002
DOI:
10.1371/journal.pntd.0010017.r003
DOI:
10.1371/journal.pntd.0010017.r004
DOI:
10.1371/journal.pntd.0010017.r005
DOI:
10.1371/journal.pntd.0010017.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2429704-5
Bookmarklink