In:
FEBS Letters, Wiley, Vol. 445, No. 2-3 ( 1999-02-26), p. 343-346
Abstract:
The rabbit polyclonal antibody against rat organic anion transporting polypeptide 2 (oatp2) was raised and immunoaffinity‐purified. Western blot analysis for oatp2 detected two bands (∼74 and 76 kDa) in rat brain and a single band (76 kDa) in the liver. By immunohistochemical analysis, the oatp2 immunoreactivity was specifically high at the basolateral membrane of rat hepatocytes. Functionally, the oatp2‐expressing oocytes were found to transport dehydroepiandrosterone sulfate, δ 1 opioid receptor agonist [ d ‐Pen 2 , d ‐Pen 5 ]enkephalin, Leu‐enkephalin, and biotin significantly, as well as the substrates previously reported. These data reveal the exact distribution of the rat oatp2 at the protein level in the liver, and that oatp2 appears to be involved in the multispecificity of the uptaking substrates in the liver and brain.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(99)00152-0
Language:
English
Publisher:
Wiley
Publication Date:
1999
detail.hit.zdb_id:
1460391-3
SSG:
12
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