In:
Traditional & Kampo Medicine, Wiley, Vol. 10, No. 2 ( 2023-08), p. 142-149
Abstract:
The purpose of this study was to explore the effects of goreisan (GRS) on rats through the analysis of 1 H‐NMR of urine samples, to assess the variations in metabolites affected by GRS, and to gain a new metabolomic understanding of the effects of Kampo medicine. Methods Male Wistar rats were randomly divided into two groups: a control group that was fed a standard diet, and a GRS group that was fed a 1% GRS diet. The study was conducted over a four‐week period. At the end of the experiment, urine samples were analyzed using 1 H‐NMR spectroscopy to evaluate the metabolic changes induced by GRS administration. Additionally, various blood parameters were also analyzed. Results Administration of GRS resulted in an elevation of lactate, acetate, and other metabolites, such as trimethylamine‐ N ‐oxide and hippurate, in urine. Additionally, there was a significant decrease in the blood urea nitrogen (BUN) level in the blood of the GRS‐treated group. Conclusion This study has highlighted the substantial changes in lactate, acetate, and other urine metabolites caused by GRS. The findings demonstrate the effectiveness of 1 H‐NMR spectroscopy in assessing metabolic alterations in response to the therapeutic application of a Kampo formula. This research contributes to the knowledge of mechanisms of action from a metabolomic point of view and offers fresh insights into the effects of complex Kampo formulas such as GRS, using 1 H‐NMR spectroscopic techniques.
Type of Medium:
Online Resource
ISSN:
2053-4515
,
2053-4515
Language:
English
Publisher:
Wiley
Publication Date:
2023
detail.hit.zdb_id:
2814360-7
Bookmarklink
