In:
Journal of Separation Science, Wiley, Vol. 45, No. 2 ( 2022-01), p. 594-601
Abstract:
In the present research, a blended polyacrylamide‐chitosan hydrogel was synthesized. For the first time, the prepared sorbent was efficiently employed in a hydrogel‐based spin‐column setup as a promising format. The proposed method was applied for monitoring the trace amounts of ketoconazole, clotrimazole, and miconazole in blood samples. Effective adsorption and desorption parameters were optimized using a central composite design and the one‐variable‐at‐a‐time method. Under the optimal conditions, the calibration curves were linear in the range of 15.0–1000.0, 1.0–1000.0, and 2.0–1000.0 ng mL –1 for ketoconazole, clotrimazole, and miconazole, respectively, along with intra‐ and interday precision less than 8.4%. Limits of detection were obtained between 0.2 and 5.0 ng mL –1 . The preconcentration factors were found in the range of 5.9–7.8. The introduced method was successfully applied for micro‐solid‐phase extraction of trace amounts of target antifungal drugs in blood samples, followed by liquid chromatography‐tandem mass spectrometry. Satisfactory relative recoveries of 94.5–103.5% were obtained, implying method reliability. Overall, the proposed method provides good accuracy and repeatability, high reusability, and good applicability to determine antifungal drugs in complex biological matrices.
Type of Medium:
Online Resource
ISSN:
1615-9306
,
1615-9314
DOI:
10.1002/jssc.202100560
Language:
English
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
2047990-6
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