In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 71, No. 6 ( 2021-06-25)
Abstract:
A novel actinobacterium, designated CFH 10395 T , was isolated from the foregut of grass carp ( Ctenopharyngodon idella ), which had been fed with ginseng extract supplement. The taxonomic position was investigated by a polyphasic approach. Cells of CFH 10395 T were Gram-staining-positive, aerobic, ovoid-shaped, non-spore-forming and non-motile. On the basis of the results of 16S rRNA gene sequence analysis, CFH 10395 T was most closely related to Brachybacterium endophyticum KCTC 49087 T , Brachybacterium squillarum JCM 16464 T and Brachybacterium paraconglomeratum JCM 17781 T (97.85%, 97.51 and 97.29% similarity, respectively). CFH 10395 T grew at 4–37 °C, pH 5.0–9.0 and in the presence of up to 10.0 % NaCl (w/v). The dominant menaquinone was MK-7. The whole-cell sugars were rhamnose, glucose, mannose and galactose. meso -diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The major fatty acids were anteiso-C 15 : 0 , anteiso-C 17 : 0 and iso-C 16 : 0 . The genome size was 3.99 Mbp with a DNA G+C content of 71.9 mol%. On the basis of the results of phylogenetic analysis, physiological properties, chemotaxonomic characteristics, low average nucleotide identity (ANI) and digital DDH (dDDH) results [ANI calculated using MUMmer (ANIm) 〈 87 %, ANI calculated using blast (ANIb) 〈 83 % and dDDH 〈 23 %], it is concluded that CFH 10395 T represents a novel species of the genus Brachybacterium , for which the name Brachybacterium subflavum sp. nov., is proposed. The type strain is CFH 10395 T (=CGMCC 1.13804 T =KCTC 49235 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.004839
Language:
English
Publisher:
Microbiology Society
Publication Date:
2021
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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