In:
Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 42, No. 5 ( 1987-5-1), p. 530-536
Abstract:
Arylsulfatase activity was detected in the green alga Chlorella fusca 211-8b during sulfur starvation. No activity was present during growth on sulfate, whereas activity was found with sulfur sources as growth-limiting factor including L-cysteine and L-methionine. Arylsulfatase activity was found after transfer to sulfate starvation conditions after a lag-period of about 3 h; addition of cycloheximide prevented arylsulfatase formation, suggesting the participation of protein synthe sis. Addition of sulfate to cultures with active arylsulfatase led to a decay of this enzyme, whereas addition of sulfate and cycloheximide prevented this decay suggesting that protein synthesis is also needed for degradation of arylsulfatase activity. The K m for p-nitrophenyl sulfate was determined to be 0.8 mм. The activity of the arylsulfatase is not affected by sulfate or phosphate, however it is inhibited strongly by sulfite, cyanide and boric acid and therefore is classified according to Dogson and Spencer (Meth. Biochem. Anal. 4, 211-254 (1957) as an arylsulfatase of type I.
Type of Medium:
Online Resource
ISSN:
1865-7125
,
0939-5075
DOI:
10.1515/znc-1987-0507
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
1987
detail.hit.zdb_id:
2078107-6
SSG:
12
Bookmarklink