In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2022-7-26), p. e0272019-
Abstract:
Coronavirus disease (COVID-19), caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is currently spreading globally. To overcome the COVID-19 pandemic, preclinical evaluations of vaccines and therapeutics using K18-hACE2 and CAG-hACE2 transgenic mice are ongoing. However, a comparative study on SARS-CoV-2 infection between K18-hACE2 and CAG-hACE2 mice has not been published. In this study, we compared the susceptibility and resistance to SARS-CoV-2 infection between two strains of transgenic mice, which were generated in FVB background mice. K18-hACE2 mice exhibited severe weight loss with definitive lethality, but CAG-hACE2 mice survived; and differences were observed in the lung, spleen, cerebrum, cerebellum, and small intestine. A higher viral titer was detected in the lungs, cerebrums, and cerebellums of K18-hACE2 mice than in the lungs of CAG-hACE2 mice. Severe pneumonia was observed in histopathological findings in K18-hACE2, and mild pneumonia was observed in CAG-hACE2. Atrophy of the splenic white pulp and reduction of spleen weight was observed, and hyperplasia of goblet cells with villi atrophy of the small intestine was observed in K18-hACE2 mice compared to CAG-hACE2 mice. These results indicate that K18-hACE2 mice are relatively susceptible to SARS-CoV-2 and that CAG-hACE2 mice are resistant to SARS-CoV-2. Based on these lineage-specific sensitivities, we suggest that K18-hACE2 mouse is suitable for highly susceptible model of SARS-CoV-2, and CAG-hACE2 mouse is suitable for mild susceptible model of SARS-CoV-2 infection.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0272019
DOI:
10.1371/journal.pone.0272019.g001
DOI:
10.1371/journal.pone.0272019.g002
DOI:
10.1371/journal.pone.0272019.g003
DOI:
10.1371/journal.pone.0272019.g004
DOI:
10.1371/journal.pone.0272019.g005
DOI:
10.1371/journal.pone.0272019.g006
DOI:
10.1371/journal.pone.0272019.g007
DOI:
10.1371/journal.pone.0272019.g008
DOI:
10.1371/journal.pone.0272019.t001
DOI:
10.1371/journal.pone.0272019.t002
DOI:
10.1371/journal.pone.0272019.s001
DOI:
10.1371/journal.pone.0272019.s002
DOI:
10.1371/journal.pone.0272019.s003
DOI:
10.1371/journal.pone.0272019.r001
DOI:
10.1371/journal.pone.0272019.r002
DOI:
10.1371/journal.pone.0272019.r003
DOI:
10.1371/journal.pone.0272019.r004
DOI:
10.1371/journal.pone.0272019.r005
DOI:
10.1371/journal.pone.0272019.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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