In:
Electroanalysis, Wiley, Vol. 18, No. 19-20 ( 2006-10), p. 1957-1962
Abstract:
An electrochemical molecular beacon aptasensor for fast and sensitive assay of thrombin is developed and characterized. A bifunctional derivative of the thrombin‐binding aptamer, 15‐base long with a redox‐active ferrocene moiety and a pendant hexanethiol linker group at the termini of the aptamer strand was immobilized on Au electrode surface. The electrochemical behavior of the aptasensor was studied using cyclic voltammetry (CV), chronoamperometry (CA), chronopotentiometry (CP), square‐wave voltammetry (SWV) and chronopotentiometric stripping analysis (PSA) techniques. Taken together, these experiments support the switch “on” mechanism of the system when the thrombin binds to the immobilized aptamer. The variation of response to the concentration of thrombin, the target protein, was evaluated by square‐wave voltammetry and chronopotentiometric stripping analysis in HEPES buffer solution (pH 8.0, 0.01 M). The aptasensor showed a specific response to thrombin in the range 1.0 to 35 nM with a detection limit of subnanomolar level concentrations. The reaction time, reproducibility, specificity and stability of the aptasensor were also studied.
Type of Medium:
Online Resource
ISSN:
1040-0397
,
1521-4109
DOI:
10.1002/elan.v18:19/20
DOI:
10.1002/elan.200603610
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
1483564-2
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