In:
Journal of Biological Physics, Springer Science and Business Media LLC, Vol. 47, No. 4 ( 2021-12), p. 521-528
Abstract:
A statistical theory is presented of the magnesium ion interacting with lysozyme under conditions where the latter is positively charged. Temporarily assuming magnesium is not noncovalently bound to the protein, I solve the nonlinear Poisson–Boltzmann equation accurately and uniformly in a perturbative fashion. The resulting expression for the effective charge, which is larger than nominal owing to overshooting, is subtle and cannot be asymptotically expanded at high ionic strengths that are practical. An adhesive potential taken from earlier work together with the assumption of possibly bound magnesium is then fitted to be in accord with measurements of the second virial coefficient by Tessier et al. The resulting numbers of bound magnesium ions as a function of MgBr $$_2$$ 2 concentration are entirely reasonable compared with densitometry measurements.
Type of Medium:
Online Resource
ISSN:
0092-0606
,
1573-0689
DOI:
10.1007/s10867-021-09587-4
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2021
detail.hit.zdb_id:
2016734-9
SSG:
12
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