In:
FEBS Letters, Wiley, Vol. 354, No. 2 ( 1994-11-07), p. 227-231
Abstract:
A series of DNA‐linked ribonucleases H with DNA adducts varying in size and sequence, ranging from heptamer to nonamer, were constructed and examined for their ability to cleave the 12‐base RNA (5′‐CGGAGAUGACGG‐3′) site‐specifically. The DNA‐linked RNase H with the 9‐base DNA (5′‐GTCATCTCC‐3′) cleaved the 12‐base RNA specifically at A6‐U7. Kinetic studies revealed that the DNA‐linked RNase H with the 8‐base DNA (5′‐TCATCTCC‐3′) cleaved it slightly more effectively than that with the 9‐base DNA. Factors that may affect the specificity and catalytic efficiency of a DNA‐linked RNase H are described.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(94)01131-1
Language:
English
Publisher:
Wiley
Publication Date:
1994
detail.hit.zdb_id:
1460391-3
SSG:
12
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