In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 10 ( 2022-10-4), p. e0274419-
Abstract:
Carbapenemase-producing Enterobacterales (CPE) are high priority targets of global antimicrobial surveillance. Herein, we determined the colonization rate of CPE on admission to intensive care units in Vientiane, Lao PDR in August-September 2019. Methods Data regarding clinical conditions, infection control, and antibiotic usage were collected during admission. Rectal swab samples (n = 137) collected during admission were inoculated to selective chromogenic agars, followed by confirmatory tests for extended-spectrum beta-lactamases and carbapenemases. All CPE isolates were sequenced on Illumina (HiSeq2500), reads assembled using SPAdes 3.13, and the draft genomes used to query a database ( https://www.genomicepidemiology.org ) for resistome, plasmid replicons, and sequence types (ST). Optical DNA mapping (ODM) was used to characterize plasmids and to determine location of resistance genes. Minimum spanning tree was generated using the Bacterial Isolate Genome Sequence database (BIGSdb) and annotated using iTOL. Result From 47 Enterobacterales isolated on selective agars, K . pneumoniae (25/47) and E . coli (12/47) were the most prevalent species, followed by K aerogenes (2/47), K . variicola (1/47), and K . oxytoca (1/47). The overall prevalence of ESBLs was 51.0%; E . coli 83.3% (10/12) and Klebsiella spp. 41.3% (12/29). Twenty percent of the K . pneumoniae (5/25) isolates were carbapenem-resistant, and 4/5 contained the bla NDM-1 gene. All bla NDM-1 isolates belonged to ST147 and were indistinguishable with cgMLST. ODM showed that the bla NDM-1 gene was located on identical plasmids in all isolates. Conclusion The prevalence of ESBL-producing Enterobacterales was high, while carbapenemases were less common. However, the detection of clonal dissemination of bla NDM-1 -producing K . pneumoniae isolates in one of the intensive care units calls for vigilance. Stringent infection prevention and antimicrobial stewardship strategies are highly important measures.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0274419
DOI:
10.1371/journal.pone.0274419.g001
DOI:
10.1371/journal.pone.0274419.g002
DOI:
10.1371/journal.pone.0274419.g003
DOI:
10.1371/journal.pone.0274419.t001
DOI:
10.1371/journal.pone.0274419.s001
DOI:
10.1371/journal.pone.0274419.s002
DOI:
10.1371/journal.pone.0274419.s003
DOI:
10.1371/journal.pone.0274419.s004
DOI:
10.1371/journal.pone.0274419.s005
DOI:
10.1371/journal.pone.0274419.s006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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