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  • 1
    Online Resource
    Online Resource
    Slovak University of Agriculture in Nitra ; 2016
    In:  Journal of microbiology, biotechnology and food sciences Vol. 5, No. special 1 ( 2016-02-08), p. 50-52
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 5, No. special 1 ( 2016-02-08), p. 50-52
    Abstract: Amygdalin is a cyanogenic glucoside initially obtained from the seeds of bitter almonds (Prunus dulcis). It is a natural product that owns antitumor activity and has also been used for the treatment of asthma, bronchitis, emphysema, leprosy and diabetes. It is composed of one molecule of benzaldehyde, two molecules of glucose and one molecule of hydrocyanic acid. Cyanide is not cancerocidal as long as glucose is available. The present in vivo study was designed to reveal whether amygdalin is able to cause changes in the energetic profile of rabbit as a biological model. A 12 adult male rabbits were randomly divided into three groups: the control group without any amygdalin administration and two experimental groups receiving daily intramuscular injections (IM) of amygdalin at 0.6 and 3.0 mg/kg b.w. respectively over the period of 28 days. The body weight of each experimental animal was recorded weekly during the whole study. Serum levels of energetic profile (glucose, triglycerides and cholesterol) were determined. Intramuscular application of amygdalin did not affect (P≥0.05) the serum levels of none of selected biochemical parameters significantly. In this in vivo study, no obvious beneficial or negative effects of amygdalin on energetic profile of male rabbits were demonstrated.
    Type of Medium: Online Resource
    ISSN: 1338-5178
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2016
    detail.hit.zdb_id: 2641525-2
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  • 2
    Online Resource
    Online Resource
    Slovak University of Agriculture in Nitra ; 2014
    In:  Journal of microbiology, biotechnology and food sciences Vol. 4, No. 3 ( 2014-12-01), p. 217-219
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 4, No. 3 ( 2014-12-01), p. 217-219
    Abstract: The aim of the study was to evaluate the effect of implementer heparin on the interaction follicles - sperm, conceptual ratio and the number of pups born in inseminated doves inseminated The experimental group was inseminated with 0.5 ml insemination dose inseminated 15 min. incubation with heparin (25 000 IU / l ml) of concentrations of 0.06 ml / 0.5 ml / ID (0.06 ml = 10 mg). The control group was inseminated with 0.5 ml insemination dose without implementer. The addition of heparin implementer has led to an increase in the reproductive performance in vitro and in vivo. Number of zygotes with two pronucleus represented in the control group 74.5% of the total number of follicles stranded in the group. In the experimental group, the number of zygotes with two pronucleus accounted for 81.08% of the total number of follicles stranded in the group. In vivo, we found a conceptual ratio 89.28% with an average number of live pups per litter 10.64 ± 3.41 pc in experimental doves. In the control group the conceptual ratio was 72.00% with an average number of pups per litter 8.55 ± 3.22 pc. The conceptual relation between the control K and experimental GAG group of doves has show a statistically significant difference (χ24, 15 +). In the number of live born pups between control K and experimental GAG group a statistically significant difference (2.02 + T41) was observed too.
    Type of Medium: Online Resource
    ISSN: 1338-5178
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2014
    detail.hit.zdb_id: 2641525-2
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  • 3
    Online Resource
    Online Resource
    Slovak University of Agriculture in Nitra ; 2017
    In:  Journal of microbiology, biotechnology and food sciences Vol. 7, No. 2 ( 2017-10-01), p. 128-131
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 7, No. 2 ( 2017-10-01), p. 128-131
    Abstract: The aim of this study was to determinate the effect of various taurine concentrations on rabbit spermatozoa in in vitro conditions with the potential use in routine artificial insemination. Taurine, amino acid distinguished by its strong antioxidant properties, has been widely tested on spermatozoa of all kinds of farm animals except for the rabbits. This study investigated differences in rabbit spermatozoa motility and viability between the control sample and samples enriched with taurine solutions: TAU3 (3.125 mM), TAU6 (6.250 mM) and TAU12 (12.5 mM). Ejaculates of nine adult rabbits were subjected to this study and observed at time intervals 0, 90, 180 and 270 minutes while cultivated at 39°C. For detection of viability the MTT test was used and motility parameters were carried out using CASA analysis. Experimental samples were compared against the control. Assessment of the mitochondrial toxicity revealed that the addition of taurine ensured the higher viability in supplemented samples however with no statistical significance. Monitored motility parameters (motility, progressive motility and velocity curved line of spermatozoa) showed that taurine treated spermatozoa have lower activity at the beginning of the study but along with increasing time the positive effect of taurine was induced. Significant (P
    Type of Medium: Online Resource
    ISSN: 1338-5178
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2017
    detail.hit.zdb_id: 2641525-2
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  • 4
    Online Resource
    Online Resource
    Slovak University of Agriculture in Nitra ; 2019
    In:  Journal of microbiology, biotechnology and food sciences Vol. 9, No. 1 ( 2019-08-01), p. 144-150
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 9, No. 1 ( 2019-08-01), p. 144-150
    Abstract: Mistletoe (Viscum album L.) was previously described as an effective additive in the therapy of patients with cancer after medical therapy of the primary neoplasia. The defence activity and also direct cytotoxic stimulation was found to be upstanding for the anti-carcinogenic action. The main target of this study was to detect the effect of Viscum album pini L. at various concentration 1.0 (PA); 0.66 (PB); 0.33 (PC); 0.1 (PD); 0.066 (PE) and 0.033 (PF) mg/mL on the fine rabbit spermatozoa motility parameters at various time periods (0; 1; 2 and 3 h) during the cultivation in vitro. The CASA methodology was applied to estimate the spermatozoa motility parameters. The spermatozoa motility at the beginning of the experiment (Time 0) found decreased rate for examined doses of Viscum album pini compared to the control, but the statistical significant reduction was observed (p
    Type of Medium: Online Resource
    ISSN: 1338-5178
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2019
    detail.hit.zdb_id: 2641525-2
    Library Location Call Number Volume/Issue/Year Availability
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  • 5
    Online Resource
    Online Resource
    Slovak University of Agriculture in Nitra ; 2018
    In:  Journal of microbiology, biotechnology and food sciences Vol. 8, No. 3 ( 2018-12-01), p. 901-904
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 8, No. 3 ( 2018-12-01), p. 901-904
    Abstract: Zinc is an essential trace element, which plays important roles in different biological activities. The aim of this study was to evaluate the effect of zinc on rabbit spermatozoa during in vitro conditions. Fresh semen was obtained from 8 sexually mature New Zealand White rabbits, which were bred at National Agricultural and Food Centre in Lužianky. Experimental groups were prepared by diluting the semen with two forms of zinc (ZnCl2, ZnSO4 .7 H2O) in five different concentration: ZA, ZA1 (4.883 mg Zn.l-1), ZB, ZB1 (9.766 mg Zn.l-1), ZC, ZC1 (19.531 mg Zn.l-1), ZD, ZD1 (39.063 mg Zn.l-1), ZE, ZE1 (78.125 mg Zn.l-1). Experimental samples were compared against control groups (ZK, ZK1). Semen was evaluated using the Computer Assisted Semen Analysis (CASA) at time intervals 0, 60, 120 and 180 minutes after incubation at 39°C. MTT test was used for determination of cell viability. Results of monitored motility parameters (motility, progressive motility and velocity curved line) showed decreasing trend in experimental samples. Significantly lower values of motility were observed in sample ZE, where percentage of motility decreased to 9.18±1.34% and after 180 minutes of incubation. Evaluation of spermatozoa velocity curved line detected significant (P
    Type of Medium: Online Resource
    ISSN: 1338-5178
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2018
    detail.hit.zdb_id: 2641525-2
    Library Location Call Number Volume/Issue/Year Availability
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