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Melatonin Lowers HIF-1α Content in Human Proximal Tubular Cells (HK-2) Due to Preventing Its Deacetylation by Sirtuin 1

Owczarek, Aleksandra ; Gieczewska, Katarzyna B. ; Polanska, Marta ; [et al.]

Frontiers Media SA ; 2021

In: Frontiers in Physiology Vol. 11 ( 2021-1-14)

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In: Frontiers in Physiology, Frontiers Media SA, Vol. 11 ( 2021-1-14)

Abstract: Although melatonin is widely known for its nephroprotective properties, there are no reports clearly pointing at its impact on the activity of hypoxia-inducible factor-1 (HIF-1), the main mediator of metabolic responses to hypoxia, in kidneys. The aim of the present study was to elucidate how melatonin affects the expression of the regulatory subunit HIF-1α in renal proximal tubules. HK-2 cells, immortalized human proximal tubular cells, were cultured under hypoxic conditions (1% O 2 ). Melatonin was applied at 100 μM concentration. Protein and mRNA contents were determined by Western blot and RT-qPCR, respectively. HIF-1α acetylation level was established by means of immunoprecipitation followed by Western blot. Melatonin receptors MT1 and MT2 localization in HK-2 cells was visualized using immunofluorescence confocal analysis. It was found that melatonin in HK-2 cells (1) lowered HIF-1α protein, but not mRNA, content; (2) attenuated expression of HIF-1 target genes; (3) increased HIF-1α acetylation level; and (4) diminished sirtuin 1 expression (both protein and mRNA). Sirtuin 1 involvement in the regulation of HIF-1α level was confirmed applying cells with silenced Sirt1 gene. Moreover, the presence of membrane MT1 and MT2 receptors was identified in HK-2 cells and their ligand, ramelteon, turned out to mimic melatonin action on both HIF-1α and sirtuin 1 levels. Thus, it is concluded that the mechanism of melatonin-evoked decline in HIF-1α content in renal proximal tubular cells involves increased acetylation of this subunit which results from the attenuated expression of sirtuin 1, an enzyme reported to deacetylate HIF-1α. This observation provides a new insight to the understanding of melatonin action in kidneys.

Type of Medium: Online Resource

ISSN: 1664-042X

URL: Article

DOI: 10.3389/fphys.2020.572911

Language: Unknown

Publisher: Frontiers Media SA

Publication Date: 2021

detail.hit.zdb_id: 2564217-0

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Risk Factors and Clinicopathological Features for Developing a Subsequent Primary Cutaneous Squamous and Basal Cell Carcinomas

Ciążyńska, Magdalena ; Pabianek, Marta ; Sławińska, Martyna ; [et al.]

MDPI AG ; 2022

In: Cancers Vol. 14, No. 13 ( 2022-06-23), p. 3069-

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In: Cancers, MDPI AG, Vol. 14, No. 13 ( 2022-06-23), p. 3069-

Abstract: Background: Patients with diagnosed keratinocyte carcinomas (KCs) have an increased risk of subsequent skin cancers development. Current studies indicate that patients with subsequent tumors should be followed up regularly. However, none of the studies indicate the connection between the specific subtypes and an increased risk for further KCs development. The study assesses the differences in the risk of developing a subsequent skin cancer after a previous diagnosis of KC, especially considering individual types of skin malignances, and identifies potential factors associated with an increased risk of new cutaneous tumor describing non-invasive diagnosis and monitoring. Methods: Pathology and medical records were examined to identify the characteristics of patients with multiple KCs diagnosed between 1999 and 2019. Results: The study group comprised 13,913 KCs occurring in 10,083 patients. Multiple KCs were observed in 2300 patients (22.8%). The analysis showed aggressive subtypes, multiple tumors, and male sex as significant prognostic factors. Conclusions: The most crucial risk factors for developing subsequent KC are being of a male gender, an aggressive tumor subtype, and previous history of multiple skin cancers. Basal cell carcinoma subtypes, such as infiltrative basosquamous, with aggressive growth patterns predispose not only to increased risk for the recurrence but are also expected to be at higher risk of subsequent KCs.

Type of Medium: Online Resource

ISSN: 2072-6694

URL: Article

DOI: 10.3390/cancers14133069

Language: English

Publisher: MDPI AG

Publication Date: 2022

detail.hit.zdb_id: 2527080-1

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Overview of Polyphenols and Polyphenol-rich Extracts as Modulators of Inflammatory Response in Dry Eye Syndrome

Caban, Miłosz ; Owczarek, Katarzyna ; Chojnacka, Katarzyna ; [et al.]

Informa UK Limited ; 2022

In: Food Reviews International Vol. 38, No. sup1 ( 2022-11-01), p. 501-528

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In: Food Reviews International, Informa UK Limited, Vol. 38, No. sup1 ( 2022-11-01), p. 501-528

Type of Medium: Online Resource

ISSN: 8755-9129 , 1525-6103

URL: Article

DOI: 10.1080/87559129.2021.1874412

Language: English

Publisher: Informa UK Limited

Publication Date: 2022

detail.hit.zdb_id: 2005738-6

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T4 Phage and Its Head Surface Proteins Do Not Stimulate Inflammatory Mediator Production

Miernikiewicz, Paulina ; Dąbrowska, Krystyna ; Piotrowicz, Agnieszka ; [et al.]

Public Library of Science (PLoS) ; 2013

In: PLoS ONE Vol. 8, No. 8 ( 2013-8-16), p. e71036-

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In: PLoS ONE, Public Library of Science (PLoS), Vol. 8, No. 8 ( 2013-8-16), p. e71036-

Type of Medium: Online Resource

ISSN: 1932-6203

URL: Article

DOI: 10.1371/journal.pone.0071036

DOI: 10.1371/journal.pone.0071036.g001

DOI: 10.1371/journal.pone.0071036.g002

DOI: 10.1371/journal.pone.0071036.g003

DOI: 10.1371/journal.pone.0071036.g004

DOI: 10.1371/journal.pone.0071036.g005

DOI: 10.1371/journal.pone.0071036.g006

DOI: 10.1371/journal.pone.0071036.g007

DOI: 10.1371/journal.pone.0071036.g008

DOI: 10.1371/journal.pone.0071036.g009

DOI: 10.1371/journal.pone.0071036.g010

DOI: 10.1371/journal.pone.0071036.g011

DOI: 10.1371/journal.pone.0071036.t001

DOI: 10.1371/journal.pone.0071036.t002

DOI: 10.1371/journal.pone.0071036.t003

DOI: 10.1371/journal.pone.0071036.t004

Language: English

Publisher: Public Library of Science (PLoS)

Publication Date: 2013

detail.hit.zdb_id: 2267670-3

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The SARS-CoV-2 ORF10 is not essential in vitro or in vivo in humans

Pancer, Katarzyna ; Milewska, Aleksandra ; Owczarek, Katarzyna ; [et al.]

Public Library of Science (PLoS) ; 2020

In: PLOS Pathogens Vol. 16, No. 12 ( 2020-12-10), p. e1008959-

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In: PLOS Pathogens, Public Library of Science (PLoS), Vol. 16, No. 12 ( 2020-12-10), p. e1008959-

Abstract: SARS-CoV-2 genome annotation revealed the presence of 10 open reading frames (ORFs), of which the last one (ORF10) is positioned downstream of the N gene. It is a hypothetical gene, which was speculated to encode a 38 aa protein. This hypothetical protein does not share sequence similarity with any other known protein and cannot be associated with a function. While the role of this ORF10 was proposed, there is growing evidence showing that the ORF10 is not a coding region. Here, we identified SARS-CoV-2 variants in which the ORF10 gene was prematurely terminated. The disease was not attenuated, and the transmissibility between humans was maintained. Also, in vitro , the strains replicated similarly to the related viruses with the intact ORF10. Altogether, based on clinical observation and laboratory analyses, it appears that the ORF10 protein is not essential in humans. This observation further proves that the ORF10 should not be treated as the protein-coding gene, and the genome annotations should be amended.

Type of Medium: Online Resource

ISSN: 1553-7374

URL: Article

DOI: 10.1371/journal.ppat.1008959

DOI: 10.1371/journal.ppat.1008959.g001

DOI: 10.1371/journal.ppat.1008959.g002

DOI: 10.1371/journal.ppat.1008959.t001

DOI: 10.1371/journal.ppat.1008959.s001

DOI: 10.1371/journal.ppat.1008959.s002

Language: English

Publisher: Public Library of Science (PLoS)

Publication Date: 2020

detail.hit.zdb_id: 2205412-1

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Canine Respiratory Coronavirus, Bovine Coronavirus, and Human Coronavirus OC43: Receptors and Attachment Factors

Szczepanski, Artur ; Owczarek, Katarzyna ; Bzowska, Monika ; [et al.]

MDPI AG ; 2019

In: Viruses Vol. 11, No. 4 ( 2019-04-05), p. 328-

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In: Viruses, MDPI AG, Vol. 11, No. 4 ( 2019-04-05), p. 328-

Abstract: Despite high similarity of canine respiratory coronavirus (CRCoV), bovine coronavirus, (BCoV) and human coronavirus OC43 (HCoV-OC43), these viruses differ in species specificity. For years it was believed that they share receptor specificity, utilizing sialic acids for cell surface attachment, internalization, and entry. Interestingly, careful literature analysis shows that viruses indeed bind to the cell surface via sialic acids, but there is no solid data that these moieties mediate virus entry. In our study, using a number of techniques, we showed that all three viruses are indeed able to bind to sialic acids to a different extent, but these molecules render the cells permissive only for the clinical strain of HCoV-OC43, while for others they serve only as attachment receptors. CRCoV and BCoV appear to employ human leukocyte antigen class I (HLA-1) as the entry receptor. Furthermore, we identified heparan sulfate as an alternative attachment factor, but this may be related to the cell culture adaptation, as in ex vivo conditions, it does not seem to play a significant role. Summarizing, we delineated early events during CRCoV, BCoV, and HCoV-OC43 entry and systematically studied the attachment and entry receptor utilized by these viruses.

Type of Medium: Online Resource

ISSN: 1999-4915

URL: Article

DOI: 10.3390/v11040328

Language: English

Publisher: MDPI AG

Publication Date: 2019

detail.hit.zdb_id: 2516098-9

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The incidence and clinical analysis of non-melanoma skin cancer

Ciążyńska, Magdalena ; Kamińska-Winciorek, Grażyna ; Lange, Dariusz ; [et al.]

Springer Science and Business Media LLC ; 2021

In: Scientific Reports Vol. 11, No. 1 ( 2021-02-22)

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In: Scientific Reports, Springer Science and Business Media LLC, Vol. 11, No. 1 ( 2021-02-22)

Abstract: Non-melanoma skin cancers (NMSCs) are the most common malignancies diagnosed in Caucasian populations. Basal cell carcinoma (BCC) is the most frequent skin cancer, followed by squamous cell carcinoma (SCC). Unfortunately, most European cancer registries do not record individual types of NMSC. To evaluate the incidence of primary BCCs and SCCs regarding age, sex, tumour site and tumour subtype to determine trends in epidemiology of both cancers. Retrospective analysis of BCCs and SCCs diagnosed and treated across seven sites in Poland from 1999 to 2019. We recorded 13,913 NMSCs occurring in 10,083 patients. BCC represented 85.2% of all cases. SCC patients were older than BCC patients (77.1 ± 11.3 years vs. 70.1 ± 12.3 years, p 〈 0.01). The nodular subtype was the most common subtype of BCC, followed by the superficial and infiltrative subtypes. The superficial BCC subtype was more common on photoprotected areas ( p 〈 0.01), whereas the nodular BCC subtype occurred on the face ( p 〈 0.01). The high-risk SCC subtypes were more common on face compared to low-risk SCC subtypes ( p 〈 0.01). BCC and SCC are common malignancies developing at various ages and anatomical sites. These data underline the need for better registration policies regarding NMSC in order to improve prevention and treatment strategies for these tumours.

Type of Medium: Online Resource

ISSN: 2045-2322

URL: Article

DOI: 10.1038/s41598-021-83502-8

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2021

detail.hit.zdb_id: 2615211-3

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Author Correction: The incidence and clinical analysis of non‑melanoma skin cancer

Ciążyńska, Magdalena ; Kamińska-Winciorek, Grażyna ; Lange, Dariusz ; [et al.]

Springer Science and Business Media LLC ; 2021

In: Scientific Reports Vol. 11, No. 1 ( 2021-07-28)

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In: Scientific Reports, Springer Science and Business Media LLC, Vol. 11, No. 1 ( 2021-07-28)

Type of Medium: Online Resource

ISSN: 2045-2322

URL: Article

DOI: 10.1038/s41598-021-94435-7

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2021

detail.hit.zdb_id: 2615211-3

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SARS-CoV-2 infects an in vitro model of the human developing pancreas through endocytosis

Szlachcic, Wojciech J. ; Dabrowska, Agnieszka ; Milewska, Aleksandra ; [et al.]

Elsevier BV ; 2022

In: iScience Vol. 25, No. 7 ( 2022-07), p. 104594-

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In: iScience, Elsevier BV, Vol. 25, No. 7 ( 2022-07), p. 104594-

Type of Medium: Online Resource

ISSN: 2589-0042

URL: Article

DOI: 10.1016/j.isci.2022.104594

Language: English

Publisher: Elsevier BV

Publication Date: 2022

detail.hit.zdb_id: 2927064-9

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Clinical factors increasing discrepancies of renal function assessment with MDRD and Cockcroft–Gault equations in old individuals

Chudek, Jerzy ; Kolonko, Aureliusz ; Owczarek, Aleksander J. ; [et al.]

Springer Science and Business Media LLC ; 2018

In: European Geriatric Medicine Vol. 9, No. 5 ( 2018-10), p. 713-720

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In: European Geriatric Medicine, Springer Science and Business Media LLC, Vol. 9, No. 5 ( 2018-10), p. 713-720

Type of Medium: Online Resource

ISSN: 1878-7657

URL: Article

DOI: 10.1007/s41999-018-0076-9

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2018

detail.hit.zdb_id: 2556794-9

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