In:
eLife, eLife Sciences Publications, Ltd, Vol. 7 ( 2018-01-29)
Abstract:
Proteins are vital for almost every process that keeps cells alive. They are made from chains of small molecules called amino acids, which need to fold into three-dimensional structures for the protein to become active. Specific molecules called chaperones help the proteins to fold properly. However, to produce proteins a lot of energy is needed. Therefore, this process is tightly coordinated with the needs of the cells to conserve energy. If too much protein is made, it can put a burden on cells and harm the organism, even when it is a protein with no apparent cellular activities. This can be a problem under stressful conditions, for example, when cells are exposed to heat or lack nutrients. For researchers who want to engineer cells to produce different or additional proteins, this poses a great challenge, as the modified cells often grow slowly or not at all. Until now, it was not known why proteins are harmful when produced in excess. To investigates this, Farkas, Kalapis et al. modified the cells of baker’s yeast to overproduce an unneeded protein. The yeast cells were then exposed to different environmental stresses, such as too much heat or lack of nutrients, and scanned for any damage. Moreover, any potential protein burden was also measured in a collection of different cells in which each lacked one dispensable gene. The results showed that when enough nutrients where present, producing too much of the protein only mildly affected cell growth. However, when exposed to different stressors, the cells grew more slowly. When Farkas, Kalapis et al. then blocked specific chaperones, the proteins could no longer fold properly and consequently, the cells became very sensitive to when the protein was produced in bulks. This study shows that chaperones or environmental stress can shape protein production costs. A next step will be to investigate how sensitive other species are to protein burden, and what the underlying molecular mechanisms might be. A better understanding of how environmental and genetic factors affect the way the organisms deal with excess proteins may help to improve engineered protein-production systems in the future.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.29845.001
DOI:
10.7554/eLife.29845.002
DOI:
10.7554/eLife.29845.003
DOI:
10.7554/eLife.29845.004
DOI:
10.7554/eLife.29845.005
DOI:
10.7554/eLife.29845.006
DOI:
10.7554/eLife.29845.007
DOI:
10.7554/eLife.29845.008
DOI:
10.7554/eLife.29845.009
DOI:
10.7554/eLife.29845.010
DOI:
10.7554/eLife.29845.011
DOI:
10.7554/eLife.29845.012
DOI:
10.7554/eLife.29845.013
DOI:
10.7554/eLife.29845.014
DOI:
10.7554/eLife.29845.015
DOI:
10.7554/eLife.29845.023
DOI:
10.7554/eLife.29845.024
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2018
detail.hit.zdb_id:
2687154-3
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