In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 1 ( 2022-1-19), p. e0262634-
Abstract:
Vigna stipulacea (Lam.) Kuntz., commonly known as Minni payaru is an underutilized legume species and has a great potential to be utilized as food crop. To evaluate and select the best germplasm to be harnessed in the breeding programme, we assessed the genetic diversity of V . stipulacea (94 accessions) conserved in the Indian National Genebank, based on morphological traits and microsatellite markers. Significant variation was recorded for the morphological traits studied. Euclidean distance using UPGMA method grouped all accessions into two major clusters. Accessions were identified for key agronomic traits such as, early flowering (IC331436, IC251436, IC331437); long peduncle length (IC553518, IC550531, IC553557, IC553540, IC550532, IC553564); and more number of seeds per pod (IC553529, IC622865, IC622867, IC553528). To analyse the genetic diversity among the germplasm 33 SSR primers were used anda total of 116 alleles were detected. The number of alleles varied from two to seven, with an average of 3.52 per loci. The polymorphic information content values varied from 0.20 to 0.74, with a mean of 0.40. The high number of alleles per locus and the allelic diversity in the studied germplasm indicated a relatively wider genetic base of V . stipulacea . Phylogenetic analysis clustered accessions into seven clades. Population structure analysis grouped them into five genetic groups, which were partly supported by PCoA and phylogenetic tree. Besides, PCoA and AMOVA also decoded high genetic diversity among the V . stipulacea accessions. Thus, morphological and microsatellite markers distinguished V . stipulacea accessions and assessed their genetic diversity efficiently. The identified promising accessions can be utilized in Vigna improvement programme through introgression breeding and/or can be used for domestication and enhanced utilization of V . stipulacea .
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0262634
DOI:
10.1371/journal.pone.0262634.g001
DOI:
10.1371/journal.pone.0262634.g002
DOI:
10.1371/journal.pone.0262634.g003
DOI:
10.1371/journal.pone.0262634.g004
DOI:
10.1371/journal.pone.0262634.g005
DOI:
10.1371/journal.pone.0262634.g006
DOI:
10.1371/journal.pone.0262634.g007
DOI:
10.1371/journal.pone.0262634.g008
DOI:
10.1371/journal.pone.0262634.t001
DOI:
10.1371/journal.pone.0262634.t002
DOI:
10.1371/journal.pone.0262634.t003
DOI:
10.1371/journal.pone.0262634.t004
DOI:
10.1371/journal.pone.0262634.t005
DOI:
10.1371/journal.pone.0262634.t006
DOI:
10.1371/journal.pone.0262634.s001
DOI:
10.1371/journal.pone.0262634.s002
DOI:
10.1371/journal.pone.0262634.s003
DOI:
10.1371/journal.pone.0262634.s004
DOI:
10.1371/journal.pone.0262634.s005
DOI:
10.1371/journal.pone.0262634.s006
DOI:
10.1371/journal.pone.0262634.s007
DOI:
10.1371/journal.pone.0262634.s008
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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