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Conjugation of Polysaccharide 6B from Streptococcus pneumoniae with Pneumococcal Surface Protein A: PspA Conformation and Its Effect on the Immune Response

Perciani, Catia T. ; Barazzone, Giovana C. ; Goulart, Cibelly ; [et al.]

American Society for Microbiology ; 2013

In: Clinical and Vaccine Immunology Vol. 20, No. 6 ( 2013-06), p. 858-866

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In: Clinical and Vaccine Immunology, American Society for Microbiology, Vol. 20, No. 6 ( 2013-06), p. 858-866

Abstract: Despite the substantial beneficial effects of incorporating the 7-valent pneumococcal conjugate vaccine (PCV7) into immunization programs, serotype replacement has been observed after its widespread use. As there are many serotypes currently documented, the use of a conjugate vaccine relying on protective pneumococcal proteins as active carriers is a promising alternative to expand PCV coverage. In this study, capsular polysaccharide serotype 6B (PS6B) and recombinant pneumococcal surface protein A (rPspA), a well-known protective antigen from Streptococcus pneumoniae , were covalently attached by two conjugation methods. The conjugation methodology developed by our laboratory, employing 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) as an activating agent through carboxamide formation, was compared with reductive amination, a classical methodology. DMT-MM-mediated conjugation was shown to be more efficient in coupling PS6B to rPspA clade 1 (rPspA1): 55.0% of PS6B was in the conjugate fraction, whereas 24% was observed in the conjugate fraction with reductive amination. The influence of the conjugation process on the rPspA1 structure was assessed by circular dichroism. According to our results, both conjugation processes reduced the alpha-helical content of rPspA; reduction was more pronounced when the reaction between the polysaccharide capsule and rPspA1 was promoted between the carboxyl groups than the amine groups (46% and 13%, respectively). Regarding the immune response, both conjugates induced functional anti-rPspA1 and anti-PS6B antibodies. These results suggest that the secondary structure of PspA1, as well as its reactive groups (amine or carboxyl) involved in the linkage to PS6B, may not play an important role in eliciting a protective immune response to the antigens.

Type of Medium: Online Resource

ISSN: 1556-6811 , 1556-679X

URL: Article

DOI: 10.1128/CVI.00754-12

Language: English

Publisher: American Society for Microbiology

Publication Date: 2013

detail.hit.zdb_id: 1496863-0

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Humoral immune response of a pneumococcal conjugate vaccine: Capsular polysaccharide serotype 14—Lysine modified PspA

Santamaria, Raquel ; Goulart, Cibelly ; Perciani, Catia T. ; [et al.]

Elsevier BV ; 2011

In: Vaccine Vol. 29, No. 47 ( 2011-11), p. 8689-8695

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In: Vaccine, Elsevier BV, Vol. 29, No. 47 ( 2011-11), p. 8689-8695

Type of Medium: Online Resource

ISSN: 0264-410X

URL: Article

DOI: 10.1016/j.vaccine.2011.08.109

Language: English

Publisher: Elsevier BV

Publication Date: 2011

detail.hit.zdb_id: 1468474-3

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Analysis of various ATP ‐binding cassette transporters revealed quantification of ABCB4 as a potential diagnostic tool in primary sclerosing cholangitis ( PSC )

Thoeni, Cornelia ; Perciani, Catia T ; Nakib, Diana ; [et al.]

Wiley ; 2023

In: Histopathology Vol. 83, No. 4 ( 2023-10), p. 559-568

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In: Histopathology, Wiley, Vol. 83, No. 4 ( 2023-10), p. 559-568

Abstract: ATP‐binding cassette transporters are important proteins in regulating bile constituent transport between hepatocytes and the bile canalicular system. Dysfunctional transporters lead to accumulation of toxic bile components within hepatocytes or the biliary system, known as cholestasis, resulting in liver damage. It has been previously reported that two particular ATP‐binding cassette transporters, ABCB4 and ABCB11, have altered expression in patients with primary sclerosing cholangitis (PSC). Interested in further analysis of expression patterns of ATP‐binding cassette transporters in PSC patients, we investigated liver samples from 201 patients, including 43 patients with PSC and 51 patients with primary biliary cholangitis patients (PBC). In addition to ABCB4 and ABCB11, we also included other ATP‐binding cassette transporters, to determine if upregulation of ABCB4 and ABCB11 is specifically found in the liver of patients with PSC. Methods and results Retrospectively, formalin‐fixed and paraffin‐embedded liver biopsies, resections, and explants were selected to investigate the expression of ABCB1 , ABCB4 , ABCB11 , ABCG5/8 , and FXR1 using nanoString nCounter and immunohistochemistry for validation of differently expressed transporters seen in PSC liver samples in comparison to non‐PSC liver specimens. Strikingly, ABCB4 was the only ATP‐binding cassette transporter showing increased gene and protein expression in hepatocytes of PSC livers when compared to non‐PSC liver specimens. Furthermore, ABCB4 protein expression also correlated with disease stage in PSC. Conclusion Our study concluded that altered ABCB4 expression is specifically seen in liver specimens of PSC patients. Therefore, quantitative ABCB4 analysis may be an additional useful tool for the histopathological diagnosis of PSC to distinguish this entity from other cholangiopathies.

Type of Medium: Online Resource

ISSN: 0309-0167 , 1365-2559

URL: Issue

URL: Article

DOI: 10.1111/his.v83.4

DOI: 10.1111/his.15006

RVK:

XA 10000

Language: English

Publisher: Wiley

Publication Date: 2023

detail.hit.zdb_id: 2006447-0

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Protection induced by pneumococcal surface protein A (PspA) is enhanced by conjugation to a Streptococcus pneumoniae capsular polysaccharide

Csordas, Fátima C.L. ; Perciani, Cátia T. ; Darrieux, Michelle ; [et al.]

Elsevier BV ; 2008

In: Vaccine Vol. 26, No. 23 ( 2008-6), p. 2925-2929

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In: Vaccine, Elsevier BV, Vol. 26, No. 23 ( 2008-6), p. 2925-2929

Type of Medium: Online Resource

ISSN: 0264-410X

URL: Article

DOI: 10.1016/j.vaccine.2008.03.038

Language: English

Publisher: Elsevier BV

Publication Date: 2008

detail.hit.zdb_id: 1468474-3

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Protocol of a randomised controlled trial characterising the immune responses induced by varicella-zoster virus (VZV) vaccination in healthy Kenyan women: setting the stage for a potential VZV-based HIV vaccine

Perciani, Catia T ; Jaoko, Walter ; Walmsley, Sharon ; [et al.]

BMJ ; 2017

In: BMJ Open Vol. 7, No. 9 ( 2017-09), p. e017391-

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In: BMJ Open, BMJ, Vol. 7, No. 9 ( 2017-09), p. e017391-

Abstract: A protective HIV vaccine would be expected to induce durable effector immune responses at the mucosa, restricting HIV infection at its portal of entry. We hypothesise that use of varicella-zoster virus (VZV) as an HIV delivery vector could generate sustained and robust tissue-based immunity against HIV antigens to provide long-term protection against HIV. Given that HIV uniquely targets immune-activated T cells, the development of human vaccines against HIV must also involve a specific examination of the safety of the vector. Thus, we aim to evaluate the effects of VZV vaccination on the recipients’ immune activation state, and on VZV-specific circulating humoral and cellular responses in addition to those at the cervical and rectal mucosa. Methods and analysis This open-label, randomised, longitudinal crossover study includes healthy Kenyan VZV-seropositive women at low risk for HIV infection. Participants receive a single dose of a commercial live-attenuated VZV Oka vaccine at either week 0 (n=22) or at week 12 (n=22) of the study and are followed for 48 and 36 weeks postvaccination, respectively. The primary outcome is the change on cervical CD4 + T-cell immune activation measured by the coexpression of CD38 and HLA-DR 12 weeks postvaccination compared with the baseline (prevaccination). Secondary analyses include postvaccination changes in VZV-specific mucosal and systemic humoral and cellular immune responses, changes in cytokine and chemokine measures, study acceptability and feasibility of mucosal sampling and a longitudinal assessment of the bacterial community composition of the mucosa. Ethics and dissemination The study has ethical approval from Kenyatta National Hospital/University of Nairobi Ethics and Research Committee, the University of Toronto Research Ethics Board and by Kenyan Pharmacy and Poisons Board. Results will be presented at conferences, disseminated to participants and stakeholders as well as published in peer-reviewed journals. Trial registration number NCT02514018. Pre-results.

Type of Medium: Online Resource

ISSN: 2044-6055 , 2044-6055

URL: Article

DOI: 10.1136/bmjopen-2017-017391

DOI: 10.1136/bmjopen-2017-017391.supp1

Language: English

Publisher: BMJ

Publication Date: 2017

detail.hit.zdb_id: 2599832-8

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Single Cell Profiling Reveals Strain-Specific Differences in Myeloid Inflammatory Potential in the Rat Liver

Pouyabahar, Delaram ; Chung, Sai W. ; Pezzutti, Olivia I. ; [et al.]

Elsevier BV ; 2022

In: SSRN Electronic Journal

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In: SSRN Electronic Journal, Elsevier BV

Type of Medium: Online Resource

ISSN: 1556-5068

URL: Article

DOI: 10.2139/ssrn.4296542

Language: English

Publisher: Elsevier BV

Publication Date: 2022

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Enhancing Immunity with Nanomedicine: Employing Nanoparticles to Harness the Immune System

Perciani, Catia T. ; Liu, Lewis Y. ; Wood, Lawrence ; [et al.]

American Chemical Society (ACS) ; 2021

In: ACS Nano Vol. 15, No. 1 ( 2021-01-26), p. 7-20

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In: ACS Nano, American Chemical Society (ACS), Vol. 15, No. 1 ( 2021-01-26), p. 7-20

Type of Medium: Online Resource

ISSN: 1936-0851 , 1936-086X

URL: Article

DOI: 10.1021/acsnano.0c08913

Language: English

Publisher: American Chemical Society (ACS)

Publication Date: 2021

detail.hit.zdb_id: 2383064-5

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Comparative analysis of DNA extraction and PCR product purification methods for cervicovaginal microbiome analysis using cpn60 microbial profiling

Shvartsman, Elinor ; Richmond, Meika E. I. ; Schellenberg, John J. ; [et al.]

Public Library of Science (PLoS) ; 2022

In: PLOS ONE Vol. 17, No. 1 ( 2022-1-13), p. e0262355-

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In: PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 1 ( 2022-1-13), p. e0262355-

Abstract: The microbiota of the lower female genital tract plays an important role in women’s health. Microbial profiling using the chaperonin 60 ( cpn 60) universal target (UT) improves resolution of vaginal species associated with negative health outcomes compared to the more commonly used 16S ribosomal DNA target. However, the choice of DNA extraction and PCR product purification methods may bias sequencing-based microbial studies and should be optimized for the sample type and molecular target used. In this study, we compared two commercial DNA extraction kits and two commercial PCR product purification kits for the microbial profiling of cervicovaginal samples using the cpn 60 UT. Methods DNA from cervicovaginal secretions and vaginal lavage samples as well as mock community standards were extracted using either the specialized QIAamp DNA Microbiome Kit, or the standard DNeasy Blood & Tissue kit with enzymatic pre-treatment for enhanced lysis of gram-positive bacteria. Extracts were PCR amplified using well-established cpn 60 primer sets and conditions. Products were then purified using a column-based method (QIAquick PCR Purification Kit) or a gel-based PCR clean-up method using the QIAEX II Gel Extraction Kit. Purified amplicons were sequenced with the MiSeq platform using standard procedures. The overall quality of each method was evaluated by measuring DNA yield, alpha diversity, and microbial composition. Results DNA extracted from cervicovaginal samples using the DNeasy Blood and Tissue kit, pre-treated with lysozyme and mutanolysin, resulted in increased DNA yield, bacterial diversity, and species representation compared to the QIAamp DNA Microbiome kit. The column-based PCR product purification approach also resulted in greater average DNA yield and wider species representation compared to a gel-based clean-up method. In conclusion, this study presents a fast, effective sample preparation method for high resolution cpn 60 based microbial profiling of cervicovaginal samples.

Type of Medium: Online Resource

ISSN: 1932-6203

URL: Article

DOI: 10.1371/journal.pone.0262355

DOI: 10.1371/journal.pone.0262355.g001

DOI: 10.1371/journal.pone.0262355.g002

DOI: 10.1371/journal.pone.0262355.g003

DOI: 10.1371/journal.pone.0262355.g004

DOI: 10.1371/journal.pone.0262355.g005

DOI: 10.1371/journal.pone.0262355.g006

DOI: 10.1371/journal.pone.0262355.t001

DOI: 10.1371/journal.pone.0262355.s001

DOI: 10.1371/journal.pone.0262355.s002

DOI: 10.1371/journal.pone.0262355.s003

DOI: 10.1371/journal.pone.0262355.s004

DOI: 10.1371/journal.pone.0262355.s005

DOI: 10.1371/journal.pone.0262355.r001

DOI: 10.1371/journal.pone.0262355.r002

DOI: 10.1371/journal.pone.0262355.r003

DOI: 10.1371/journal.pone.0262355.r004

Language: English

Publisher: Public Library of Science (PLoS)

Publication Date: 2022

detail.hit.zdb_id: 2267670-3

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Examining the Species-Specificity of Rhesus Macaque Cytomegalovirus (RhCMV) in Cynomolgus Macaques

Marsh, Angie K. ; Ambagala, Aruna P. ; Perciani, Catia T. ; [et al.]

Public Library of Science (PLoS) ; 2015

In: PLOS ONE Vol. 10, No. 3 ( 2015-3-30), p. e0121339-

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In: PLOS ONE, Public Library of Science (PLoS), Vol. 10, No. 3 ( 2015-3-30), p. e0121339-

Type of Medium: Online Resource

ISSN: 1932-6203

URL: Article

DOI: 10.1371/journal.pone.0121339

DOI: 10.1371/journal.pone.0121339.g001

DOI: 10.1371/journal.pone.0121339.g002

DOI: 10.1371/journal.pone.0121339.g003

DOI: 10.1371/journal.pone.0121339.g004

DOI: 10.1371/journal.pone.0121339.g005

DOI: 10.1371/journal.pone.0121339.t001

DOI: 10.1371/journal.pone.0121339.t002

DOI: 10.1371/journal.pone.0121339.t003

DOI: 10.1371/journal.pone.0121339.s001

DOI: 10.1371/journal.pone.0121339.s002

Language: English

Publisher: Public Library of Science (PLoS)

Publication Date: 2015

detail.hit.zdb_id: 2267670-3

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Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

Chen, Xu-Chun ; Sekhon, Manmeet ; Ma, Xue-Zhong ; [et al.]

MyJove Corporation ; 2020

In: Journal of Visualized Experiments , No. 165 ( 2020-11-07)

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In: Journal of Visualized Experiments, MyJove Corporation, , No. 165 ( 2020-11-07)

Type of Medium: Online Resource

ISSN: 1940-087X

URL: Article

DOI: 10.3791/60628-v

Language: English

Publisher: MyJove Corporation

Publication Date: 2020

detail.hit.zdb_id: 2259946-0

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