In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 100, No. 1 ( 2003-01-07), p. 318-323
Abstract:
Microarrays comprise an efficient approach to discovering large numbers of differentially expressed mRNA transcripts in the CNS resulting from changes in hormonal milieu. We used high-density oligonucleotide microarrays to examine the short- and long-term actions of estradiol (E 2 ) on the transcriptomes from the medial basal hypothalamus and other brain regions of E 2 -treated (10 μg) adult female mice. Our results have revealed several unanticipated gene regulations. Most striking is lipocalin prostaglandin D 2 synthase (L-PGDS), which catalyzes the conversion of prostaglandin (PG) H 2 to PGD 2 , a neuromodulator involved in a variety of functions, including sleep, pain, and odor responses. In situ hybridization revealed significant increases in L-PGDS expression in the arcuate and ventromedial nucleus of the medial basal hypothalamus compared with vehicle controls. The magnitude of these changes is ≈2-fold and suggests a modulatory role for PGD 2 in E 2 -controlled neuroendocrine secretions and behaviors. Surprisingly, L-PGDS gene expression is reduced 2-fold after E 2 treatment in the ventrolateral preoptic area (VLPO), the suspected site of action for the sleep-promoting effects of PGD 2 . Finally, whereas L-PGDS has been reported to be expressed primarily in oligodendrocytes of the adult rodent brain, we demonstrate, immunocytochemically, that L-PGDS is also expressed in a population of VLPO neurons. Thus, our data suggest the intriguing possibility that E 2 modulation of L-PGDS plays a role in the regulation of sleep–wake states through hitherto unknown mechanisms in VLPO neurons and through hormone-dependent neuronal-glial cooperation.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.262663799
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2003
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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