In:
Intervirology, S. Karger AG, Vol. 41, No. 2-3 ( 1998), p. 80-90
Abstract:
Human papillomavirus 8 (HPV8) is one of the oncogenic HPV types specifically associated with skin cancers of epidermodysplasia verruciformis patients. The early gene products of this virus exert functions in transformation (E2, E6, E7), replication (E1, E2) and in the control of viral transcription (E2, E7). Many viral and cellular transactivators of transcription have been shown to interact selectively and directly with a number of TATA-box-binding protein (TBP)-associated factors (TAF 〈 sub 〉 II 〈 /sub 〉 s), which then play a role as coactivators. Using glutathione-S-transferase (GST) pull-down experiments, we tested in vitro interactions between GST-HPV8-E1, -E2, -E6 and -E7 and 7 in-vitro-translated TAF 〈 sub 〉 II 〈 /sub 〉 s in the human (h) system (hTAF 〈 sub 〉 II 〈 /sub 〉 18, hTAF 〈 sub 〉 II 〈 /sub 〉 20, hTAF 〈 sub 〉 II 〈 /sub 〉 28, hTAF 〈 sub 〉 II 〈 /sub 〉 30, hTAF 〈 sub 〉 II 〈 /sub 〉 55, hTAF 〈 sub 〉 II 〈 /sub 〉 100, hTAF 〈 sub 〉 II 〈 /sub 〉 ΔN135) or TBP. We could show that GST-HPV8-E2 interacts directly at least with hTAF 〈 sub 〉 II 〈 /sub 〉 55 and TBP. Deletion analysis indicated that a domain overlapping with the C-terminal moiety of HPV8-E2 is required for binding to TBP, whereas determinants for interactions with hTAF 〈 sub 〉 II 〈 /sub 〉 55 are in the central and C-terminal part of the E2 protein. In similar binding studies, GST-HPV8-E6 interacted with hTAF 〈 sub 〉 II 〈 /sub 〉 28, hTAF 〈 sub 〉 II 〈 /sub 〉 ΔN135 and TBP, and more weakly with hTAF 〈 sub 〉 II 〈 /sub 〉 20, whereas GST- HPV8-E7 bound to hTAF 〈 sub 〉 II 〈 /sub 〉 20, hTAF 〈 sub 〉 II 〈 /sub 〉 28, hTAF 〈 sub 〉 II 〈 /sub 〉 55, hTAF 〈 sub 〉 II 〈 /sub 〉 ΔN135 and TBP. Deletion analysis revealed that the C-terminal part of HPV8-E7 is required for the interaction with these hTAF 〈 sub 〉 II 〈 /sub 〉 s. In contrast, no interactions were observed between GST-HPV8-E1 and in-vitro-translated hTAF 〈 sub 〉 II 〈 /sub 〉 s.
Type of Medium:
Online Resource
ISSN:
0300-5526
,
1423-0100
Language:
English
Publisher:
S. Karger AG
Publication Date:
1998
detail.hit.zdb_id:
1482863-7
SSG:
12
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