In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 83, No. 7_Supplement ( 2023-04-04), p. 5146-5146
Abstract:
Neutrophils have shown a lot of plasticity with important consequences on cancer disease progression and have been associated with multiple functions such as killing antibody-opsonized cancer cells, directly kill tumor cells through the release of reactive oxygen and nitrogen species and a controversial role in the tumor micro-environment with both pro- and anti-tumor roles. Studying neutrophil function in vitro can be done using the Incucyte® live imaging system to monitor antibody-dependent cell-mediated cytotoxicity (ADCC). Optimised in vitro assays using fresh human neutrophils and fluorescent-labeled tumor cells can be used to screen the potency of candidate therapeutics. Additionally, neutrophil chemotaxis can be monitored using transwell plates and live imaging via Incucyte®. Finally, multiplex cytokine and chemokine analysis after activation and stimulation of neutrophils can be evaluated using Luminex technology. When studying neutrophil activity in vitro, important factors such as access to fresh blood and optimized protocols for purifying untouched neutrophils are key for reproducible and reliable results. Citation Format: Alanah Pieters, Martijn Vlaming, Jezabel Lefevere, Sofie Pattyn. In vitro neutrophil assays to support immuno-oncology drug development. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 5146.
Type of Medium:
Online Resource
ISSN:
1538-7445
DOI:
10.1158/1538-7445.AM2023-5146
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2023
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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