In:
PLOS Biology, Public Library of Science (PLoS), Vol. 20, No. 11 ( 2022-11-15), p. e3001870-
Abstract:
Bunyaviruses lack a specific mechanism to ensure the incorporation of a complete set of genome segments into each virion, explaining the generation of incomplete virus particles lacking one or more genome segments. Such incomplete virus particles, which may represent the majority of particles produced, are generally considered to interfere with virus infection and spread. Using the three-segmented arthropod-borne Rift Valley fever virus as a model bunyavirus, we here show that two distinct incomplete virus particle populations unable to spread autonomously are able to efficiently complement each other in both mammalian and insect cells following co-infection. We further show that complementing incomplete virus particles can co-infect mosquitoes, resulting in the reconstitution of infectious virus that is able to disseminate to the mosquito salivary glands. Computational models of infection dynamics predict that incomplete virus particles can positively impact virus spread over a wide range of conditions, with the strongest effect at intermediate multiplicities of infection. Our findings suggest that incomplete particles may play a significant role in within-host spread and between-host transmission, reminiscent of the infection cycle of multipartite viruses.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001870
DOI:
10.1371/journal.pbio.3001870.g001
DOI:
10.1371/journal.pbio.3001870.g002
DOI:
10.1371/journal.pbio.3001870.g003
DOI:
10.1371/journal.pbio.3001870.g004
DOI:
10.1371/journal.pbio.3001870.g005
DOI:
10.1371/journal.pbio.3001870.g006
DOI:
10.1371/journal.pbio.3001870.g007
DOI:
10.1371/journal.pbio.3001870.g008
DOI:
10.1371/journal.pbio.3001870.t001
DOI:
10.1371/journal.pbio.3001870.s001
DOI:
10.1371/journal.pbio.3001870.s002
DOI:
10.1371/journal.pbio.3001870.s003
DOI:
10.1371/journal.pbio.3001870.s004
DOI:
10.1371/journal.pbio.3001870.s005
DOI:
10.1371/journal.pbio.3001870.s006
DOI:
10.1371/journal.pbio.3001870.s007
DOI:
10.1371/journal.pbio.3001870.s008
DOI:
10.1371/journal.pbio.3001870.s009
DOI:
10.1371/journal.pbio.3001870.s010
DOI:
10.1371/journal.pbio.3001870.s011
DOI:
10.1371/journal.pbio.3001870.s012
DOI:
10.1371/journal.pbio.3001870.s013
DOI:
10.1371/journal.pbio.3001870.s014
DOI:
10.1371/journal.pbio.3001870.s015
DOI:
10.1371/journal.pbio.3001870.r001
DOI:
10.1371/journal.pbio.3001870.r002
DOI:
10.1371/journal.pbio.3001870.r003
DOI:
10.1371/journal.pbio.3001870.r004
DOI:
10.1371/journal.pbio.3001870.r005
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2126773-X
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