In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 9 ( 2023-9-28), p. e0291146-
Abstract:
With the onset of COVID-19, the development of ex vivo laboratory models became an urgent priority to study host-pathogen interactions in response to the pandemic. In this study, we aimed to establish an ex vivo mucosal tissue explant challenge model for studying SARS-CoV-2 infection and replication. Nasal or oral tissue samples were collected from eligible participants and explants generated from the tissue were infected with various SARS-CoV-2 strains, including IC19 (lineage B.1.13), Beta (lineage B.1.351) and Delta (lineage B.1.617.2). A qRT-PCR assay used to measure viral replication in the tissue explants over a 15-day period, demonstrated no replication for any viral strains tested. Based on this, the ex vivo challenge protocol was modified by reducing the viral infection time and duration of sampling. Despite these changes, viral infectivity of the nasal and oral mucosa was not improved. Since 67% of the enrolled participants were already vaccinated against SARS-CoV-2, it is possible that neutralizing antibodies in explant tissue may have prevented the establishment of infection. However, we were unable to optimize plaque assays aimed at titrating the virus in supernatants from both infected and uninfected tissue, due to limited volume of culture supernatant available at the various collection time points. Currently, the reasons for the inability of these mucosal tissue samples to support replication of SARS-CoV-2 ex vivo remains unclear and requires further investigation.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0291146
DOI:
10.1371/journal.pone.0291146.g001
DOI:
10.1371/journal.pone.0291146.g002
DOI:
10.1371/journal.pone.0291146.t001
DOI:
10.1371/journal.pone.0291146.s001
DOI:
10.1371/journal.pone.0291146.s002
DOI:
10.1371/journal.pone.0291146.s003
DOI:
10.1371/journal.pone.0291146.s004
DOI:
10.1371/journal.pone.0291146.s005
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10.1371/journal.pone.0291146.s006
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10.1371/journal.pone.0291146.s007
DOI:
10.1371/journal.pone.0291146.s008
DOI:
10.1371/journal.pone.0291146.s009
DOI:
10.1371/journal.pone.0291146.s010
DOI:
10.1371/journal.pone.0291146.s011
DOI:
10.1371/journal.pone.0291146.s012
DOI:
10.1371/journal.pone.0291146.s013
DOI:
10.1371/journal.pone.0291146.s014
DOI:
10.1371/journal.pone.0291146.s015
DOI:
10.1371/journal.pone.0291146.s016
DOI:
10.1371/journal.pone.0291146.s017
DOI:
10.1371/journal.pone.0291146.r001
DOI:
10.1371/journal.pone.0291146.r002
DOI:
10.1371/journal.pone.0291146.r003
DOI:
10.1371/journal.pone.0291146.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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