In:
Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 56, No. 4 ( 2012-04), p. 1693-1697
Abstract:
The carbapenemase NDM-1 has been identified recently in Enterobacteriaceae and Acinetobacter baumannii as a source of multidrug resistance, including resistance to carbapenems. By analyzing the immediate genetic environment of the bla NDM-1 carbapenemase gene among a series of NDM-1-producing enterobacterial isolates, a novel gene ( ble MBL , for ble gene associated with the metallo-β-lactamase NDM-1) was identified. The ble MBL gene encodes a novel bleomycin resistance protein (BRP), named BRP MBL , that shares weak similarities with known BRPs (less than 60% amino acid identity). The expression of BRP MBL conferred resistance to bleomycin and to bleomycin-like molecules in Enterobacteriaceae and A. baumannii . The bla NDM-1 and ble MBL genes were coexpressed under the control of the same promoter, located upstream of the bla NDM-1 gene and at the extremity of the insertion sequence IS Aba125 . Most of the NDM producers possessed the ble MBL gene. Although BRP MBL did not modify the growth or death rates of Escherichia coli under experimental conditions, it suppressed the mutation rate of hypermutable E. coli and therefore may stabilize the plasmid-borne bla NDM-1 gene. This study suggests that the emerging carbapenemase NDM-1 is selected by bleomycin-like molecules, and that BRP MBL producers (and consequently NDM producers) are better suited to various environments.
Type of Medium:
Online Resource
ISSN:
0066-4804
,
1098-6596
DOI:
10.1128/AAC.05583-11
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2012
detail.hit.zdb_id:
1496156-8
SSG:
12
SSG:
15,3
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