In:
Pharmaceutical Research, Springer Science and Business Media LLC, Vol. 37, No. 4 ( 2020-04)
Abstract:
Development of zeta potential changing SEDDS containing newly synthesized derivative stearic acid phosphotyrosine amide. Methods Stearoyl chloride was conjugated with phosphotyrosine, which is substrate for the brush border enzyme intestinal alkaline phosphate. The synthesized derivative was implemented in different SEDDS formulations and the zeta potential changing properties and the concluding mucus diffusion abilities were evaluated. Results Stearic acid phosphotyrosine amide was successfully synthesized and incorporated into SEDDS. A SEDDS formulation containing the new derivative showed a zeta potential of −14 mV before, and + 2 mV after enzymatic cleavage by intestinal alkaline phosphatase. Experiments on a Caco-2 monolayer demonstrated that the phosphate cannot only be cleaved by isolated enzyme, but also by enzyme, which was expressed by cells. The mucus diffusion abilities of the untreated, negatively charged SEDDS were significantly higher compared to the enzymatically cleaved, positively charged SEDDS. Conclusion The developed stearic acid phosphotyrosine represents a promising excipient for zeta potential changing SEDDS.
Type of Medium:
Online Resource
ISSN:
0724-8741
,
1573-904X
DOI:
10.1007/s11095-020-02802-2
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2020
detail.hit.zdb_id:
2036232-8
SSG:
15,3
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