In:
Journal of Separation Science, Wiley, Vol. 26, No. 9-10 ( 2003-07), p. 771-776
Abstract:
A stereospecific gas chromatography (GC) method using a (6‐ O ‐ t butyldimethylsilyl‐2,3‐di‐ O ‐methyl)‐β‐cyclodextrin as the chiral stationary phase has been developed and validated for the determination of the enantiomers of 1,2‐ O ‐isopropylidene‐sn‐glycerol (IPG), an important chiral synthon, in kinetic resolution experiments. After liquid‐liquid extraction with ethyl acetate, the enantiomers of R ‐(–)‐IPG and S ‐(+)‐IPG and their corresponding butyrate‐ and caprylate esters were baseline separated using this chiral GC method. The method was accurate, reproducible, specific, and precise and recoveries of at least 77% were accomplished. The detection limits for IPG and its esters were 0.6 ng and 0.1 ng, respectively. The application of this method was demonstrated by a kinetic resolution experiment using the lipase A of Bacillus subtilis .
Type of Medium:
Online Resource
ISSN:
1615-9306
,
1615-9314
DOI:
10.1002/jssc.v26:9/10
DOI:
10.1002/jssc.200301509
Language:
English
Publisher:
Wiley
Publication Date:
2003
detail.hit.zdb_id:
2047990-6
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