In:
European Journal of Neuroscience, Wiley, Vol. 24, No. 5 ( 2006-09), p. 1395-1403
Abstract:
Much of our knowledge on trafficking of neurotransmitter receptors derives from heterologous expression systems and neurons in vitro . Understanding these dynamics in vivo for dopamine receptors, and D 2 receptors (D 2 Rs) in particular, presents a foremost challenge as their pharmacological manipulation underlies antipsychotic medications and drug abuse, which may in turn alter response to endogenous dopamine. Here we present the first ultrastructural evidence of clathrin‐mediated endocytosis of D 2 Rs or any other neurotransmitter receptor in the primate brain. We have captured in situ the insertion of D 2 Rs in clathrin‐coated membrane pits, resulting in receptor sorting in primary endosomes. Endocytosis was specific to nonsynaptic membranes of distal dendrites, and virtually absent from larger shafts, spines, axons and perikarya expressing D 2 Rs. The selective association of D 2 Rs with the clathrin endocytotic pathway of high‐order dendrites identifies a novel substrate for monitoring and adjusting dopaminoception, as well as a potent target for dysregulation, and manipulation, of D 2 R signalling in mental illness.
Type of Medium:
Online Resource
ISSN:
0953-816X
,
1460-9568
DOI:
10.1111/ejn.2006.24.issue-5
DOI:
10.1111/j.1460-9568.2006.05023.x
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
2005178-5
SSG:
12
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