In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 4 ( 2021-4-9), p. e0245143-
Abstract:
Tumor necrosis factor (TNF)α is an inflammatory cytokine likely to be involved in the process of corneal inflammation and neovascularization. In the present study we evaluate the role of the two receptors, TNF-receptor (TNF-R)p55 and TNF-Rp75, in the mouse model of suture-induced corneal neovascularization and lymphangiogenesis. Corneal neovascularization and lymphangiogenesis were induced by three 11–0 intrastromal corneal sutures in wild-type (WT) C57BL/6J mice and TNF-Rp55-deficient ( TNF-Rp55d ) and TNF-Rp75-deficient ( TNF-Rp75d ) mice. The mRNA expression of VEGF-A, VEGF-C, Lyve-1 and TNFα and its receptors was quantified by qPCR. The area covered with blood- or lymphatic vessels, respectively, was analyzed by immunohistochemistry of corneal flatmounts. Expression and localization of TNFα and its receptors was assessed by immunohistochemistry of sagittal sections and Western Blot. Both receptors are expressed in the murine cornea and are not differentially regulated by the genetic alteration. Both TNF-Rp55d and TNF-Rp75d mice showed a decrease in vascularized area compared to wild-type mice 14 days after suture treatment. After 21 days there were no differences detectable between the groups. The number of VEGF-A-expressing macrophages did not differ when comparing WT to TNF-Rp55d and TNF-Rp75d . The mRNA expression of lymphangiogenic markers VEGF-C or LYVE-1 does not increase after suture in all 3 groups and lymphangiogenesis showed a delayed effect only for TNF-Rp75d . TNFα mRNA and protein expression increased after suture treatment but showed no difference between the three groups. In the suture-induced mouse model, TNFα and its ligands TNF-Rp55 and TNF-Rp75 do not play a significant role in the pathogenesis of neovascularisation and lymphangiogenesis.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0245143
DOI:
10.1371/journal.pone.0245143.g001
DOI:
10.1371/journal.pone.0245143.g002
DOI:
10.1371/journal.pone.0245143.g003
DOI:
10.1371/journal.pone.0245143.g004
DOI:
10.1371/journal.pone.0245143.t001
DOI:
10.1371/journal.pone.0245143.s001
DOI:
10.1371/journal.pone.0245143.s002
DOI:
10.1371/journal.pone.0245143.s003
DOI:
10.1371/journal.pone.0245143.s004
DOI:
10.1371/journal.pone.0245143.s005
DOI:
10.1371/journal.pone.0245143.s006
DOI:
10.1371/journal.pone.0245143.s007
DOI:
10.1371/journal.pone.0245143.s008
DOI:
10.1371/journal.pone.0245143.r001
DOI:
10.1371/journal.pone.0245143.r002
DOI:
10.1371/journal.pone.0245143.r003
DOI:
10.1371/journal.pone.0245143.r004
DOI:
10.1371/journal.pone.0245143.r005
DOI:
10.1371/journal.pone.0245143.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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